DIETARY LIPIDS--EFFECTS ON LIPID-LIPOPROTEIN METABOLISM

我们提出了一个综合系列的临床和生化调查 为了更好地描述特定膳食脂质对脂质的影响， 在人类受试者中的脂蛋白代谢。 饮食的影响， 单不饱和脂肪酸代表脂肪的主要来源将是 研究了它们的降脂作用，与类似的 饮食中含有30%的热量来自omega-6多不饱和脂肪酸 或主要是饱和脂肪酸，这两种脂肪酸与低脂肪相比， 饮食. 所有饮食均将用于原发性高胆固醇血症受试者 研究的脂蛋白代谢参数包括 125 I-LDL转换，通过血清和尿液评估胆固醇稳态 新鲜分离的肝细胞中甲羟戊酸的排泄和胆固醇的合成 单核白细胞 这四种饮食将被喂给食用 不含胆固醇的饮食，并且在存在600 mg/天的膳食 胆固醇 长链多不饱和脂肪酸的代谢效应 鱼油衍生酸将在项目概述的研究中进行检查。 2. 这些研究的目的是检查是否低血糖的影响， 长链ω-3多不饱和脂肪酸主要是由于 二十碳五烯酸或二十二碳六烯酸， 纯化的脂肪酸（作为膳食成分）可防止碳水化合物诱导的 高胆固醇血症 降低HDL的机制 高胆固醇血症受试者喂食低胆固醇鱼的浓度 通过用125 I标记的HDL进行动力学研究来检查油。 鱼油对前列腺素类形成的影响将在 合作研究。 这些研究应该能增进我们的知识 关于长链ω-3脂肪酸的抗动脉粥样硬化作用 acids. 甾醇对血脂及脂蛋白的影响 将在正常和高胆固醇血症受试者中检查浓度 喂食补充有海鲜固醇的饮食。 最后，第四个项目 目的是检查三个阶段的降胆固醇效果， 在志愿者受试者中谨慎饮食，建立食谱开发 实验室，并前瞻性地评估饮食的有效性， 咨询作为长期控制血脂的辅助手段， 门诊设置。 总的来说，这些研究应该提高我们的知识， 关于特定饮食脂质的临床和代谢作用 并具有直接的治疗应用， 高胆固醇血症

We propose an integrated series of clinical and biochemical investigations to better delineate the effects of specific dietary lipids on lipid and lipoprotein metabolism in human subjects. The influence of diets in which monounsaturated fatty acids represent the main source of fat will be investigated in terms of their lipid lowering effects compared to similar diets containing 30% of calories from omega-6 polyunsaturated fatty acids or primarily saturated fatty acids and both of these compared to a low fat diet. All diets will be fed to subjects with primary hypercholesterolemia and parameters of lipoprotein metabolism to be studied will include 125I-LDL turnover, cholesterol homeostasis assessed by serum and urinary excretion of mevalonic acid and cholesterol synthesis in freshly isolated mononuclear leukocytes. These four diets will be fed to subjects consuming a cholesterol-free diet and in the presence of 600 mg/day of dietary cholesterol. The metabolic effects of long chain polyunsaturated fatty acids derived from fish oil will be examined in studies outlined in Project 2. These studies aim to examine whether the hypotriglyceridemic effects of long-chain omega-3 polyunsaturated fatty acids are due primarily to eicosapentaenoic acid or docosahexaenoic acid by determining if these purified fatty acids (as dietary constituents) prevent carbohydrate induced hypertriglyceridemia. The mechanisms responsible for the reduction of HDL concentrations in hypercholesterolemic subjects fed a low cholesterol fish oil will be examined by performing kinetic studies with 125I-labeled HDL. The influence of fish oils on prostanoid formation will be examined in collaborative studies. These studies should further our knowledge concerning the anti-atherosclerotic effects of long chain omega-3 fatty acids. The influence of sterols on plasma lipid and lipoprotein concentrations will be examined in normal and hypercholesterolemic subjects fed diets supplemented with seafood sterols. Finally, the fourth project aims to examine the hypocholesterolemic effects of three phases of a prudent diet in volunteer subjects, establish a recipe development laboratory, and prospectively assess the effectiveness of dietary counseling as an adjunct to long-term control of plasma lipids in an outpatient setting. Overall, these studies should enhance our knowledge concerning the clinical and metabolic effects of specific dietary lipids and have direct therapeutic application to treatment of subjects with hypercholesterolemia.