ASSOCIATION OF H-2 AND LUNG DEVELOPMENT

哺乳动物主要组织相容性复合体(MHC)(小鼠的H-2； 人类的人类白细胞抗原)是一个多基因家族，其成员编码细胞- 表面糖蛋白。最近，人们一直在关注。专注于 MHC相关基因可能的非免疫功能。这是众所周知的 存在一个或多个与H-2相关的主要基因 影响植入前后发育的复合体 包括第一次卵裂分裂的时间，类固醇受体 下颌骨的数量和反应性、形态发生及其他 面部结构和整个胎儿的生长和存活。变异 所有这些性状都与H-2单倍型变异有关。 我们实验室的初步研究表明，情况也是如此 对于正常的肺部发育也是如此。鉴于此，我们将使用 形态计量分析，以及特定的育种方案和数据 分析以确定(A)肺的关联度 H-2单倍型的发育，(B)母体的存在 影响(基因型、细胞质等)，以及(C) 同源菌株间的总表型变异 对环境变化的影响。此外，我们将使用单克隆 抗体以确定特定的时空模式是否 H-2在小鼠肺发育过程中的定位相同或不同 具有不同单倍型的同源基因小鼠。因为皮质类固醇 都会加速肺部发育，我们将重复所有 实验后用相同的母体处理。

The major histocompatibility complex (MHC) of mammals (H-2 in mice; HLA in humans) is a multigene family whose members encode cell - surface glycoproteins. Recently, attention has been. focused on possible non-immune functions of MHC associated genes. It is known that there are one or more major genes associated with the H-2 complex that influence pre- and post-implantation development including timing of the first cleavage division, steroid receptor number and responsiveness, morphogenesis of the mandible and other facial structures and overall fetal growth and survival. Variation in all of these traits is related to variation in H-2 haplotype. Preliminary studies in our laboratory suggests that the same is true of normal lung development. Given this, we will use morphometric assays, along with specific breeding schemes and data analyses to determine (a) the degree of association of lung development with H-2 haplotype, (b) the existence of maternal effects (genotype cytoplasmic, etc.), and (c) the proportion of the total phenotypic variation among congenic strains that is due to environmental variation. Further, we will use monoclonal antibodies to determine if the specific spatiotemporal patterns of H-2 localization are the same or different in developing lungs of congenic mice with differing haplotypes. Since corticosteroids are known to accelerate lung development, we will repeat all experiments after maternal treatment with same.