ASSOCIATION OF H-2 AND LUNG DEVELOPMENT

哺乳动物的主要组织相容性复合体（MHC）（小鼠中的H-2; 人类的HLA）是一个多基因家族，其成员编码细胞- 表面糖蛋白 最近，人们注意到。专注于 MHC相关基因可能的非免疫功能。 已知 有一个或多个与H-2相关的主基因 影响着床前和着床后发育的复合体 包括第一次卵裂分裂的时间，类固醇受体 数量和反应性，下颌骨的形态发生和其他 面部结构和整体胎儿的生长和存活。 变化 在所有这些性状中，都与H-2单倍型的变异有关。 我们实验室的初步研究表明， 肺的正常发育。 鉴于此，我们将使用 形态测定，沿着特定的育种方案和数据 分析以确定（a）肺的相关性程度 （B）母体的存在 影响（基因型细胞质等），及（c）以下各项的比例 同类菌株间的总表型变异， to environmental环境variation变化.此外，我们将使用单克隆 抗体，以确定特定的时空模式， H-2在肺发育过程中的定位是相同或不同的， 具有不同单倍型的同类小鼠。 因为皮质类固醇 会加速肺部发育，我们将重复所有 在用相同的药物进行母体处理后进行实验。

The major histocompatibility complex (MHC) of mammals (H-2 in mice; HLA in humans) is a multigene family whose members encode cell - surface glycoproteins. Recently, attention has been. focused on possible non-immune functions of MHC associated genes. It is known that there are one or more major genes associated with the H-2 complex that influence pre- and post-implantation development including timing of the first cleavage division, steroid receptor number and responsiveness, morphogenesis of the mandible and other facial structures and overall fetal growth and survival. Variation in all of these traits is related to variation in H-2 haplotype. Preliminary studies in our laboratory suggests that the same is true of normal lung development. Given this, we will use morphometric assays, along with specific breeding schemes and data analyses to determine (a) the degree of association of lung development with H-2 haplotype, (b) the existence of maternal effects (genotype cytoplasmic, etc.), and (c) the proportion of the total phenotypic variation among congenic strains that is due to environmental variation. Further, we will use monoclonal antibodies to determine if the specific spatiotemporal patterns of H-2 localization are the same or different in developing lungs of congenic mice with differing haplotypes. Since corticosteroids are known to accelerate lung development, we will repeat all experiments after maternal treatment with same.