NEURAL SUBSTRATE OF BEHAVIORAL DEVELOPMENT

将使用神经解剖学、行为学和生化方法来 大鼠侧嗅束再生系统的特征 仓鼠。总体目的是确定为什么 再神经支配的尾部嗅觉皮质在7日龄后消失。 在单个幼崽身上进行的解剖学和行为学联合实验将 确定嗅结节的再神经支配是否与 嗅觉导引探查和社会功能恢复的时间 行为。少量的HRP沉淀物将被放置在嗅球内 不同年龄和单个轴突的束切片将被追踪到 为了确定损伤区域是否长时间发射概率 络脉及其成功穿越破损区域的变化 年龄。一些有HRP沉积的病例将用 用电子显微镜来确定再生芽是否会 与结缔组织元素(PIA和血液)的特殊接触 血管)和神经胶质细胞，或者它们是否采取任何特定的路线穿过伤疤。 该区域的生化特征将决定是否 神经丝的组成随年龄的变化以及神经丝的稳定性 神经束切面后的神经丝在第7天后发生变化。 这些实验的结果将有助于阐明细胞机制。 负责损伤后和损伤过程中的功能神经重建 正常发育。

Neuroanatomical, behavioral, and biochemical methods will be used to characterize the regenerating lateral olfactory tract system of the hamster. The overall purpose is to determine why the capacity to reinnervate caudal olfactory cortex is lost after the age of 7 days. Combined anatomical and behavioral experiments in individual pups will determine whether reinnervation of the olfactory tubercle correlates in time with functional recovery of olfactory guided exploration and social behavior. Small deposits of HRP will be placed in the olfactory bulb after tract section at different ages and single axons will be traced into the region of damage in order to determine if the probability of emitting long collaterals and their success in crossing the damaged region changes with age. Some cases with HRP deposits will be examined with the electronmicroscope to determine if the regenerating sprouts make specialized contacts with connective tissue elements (pia and blood vessels) and glia or whether they take any specific routes across the scar. Biochemical characterization of the tract region will determine whether the neurofilament composition changes with age and whether the stability of the neurofilaments after tract section changes after day 7. It is hoped that the results of these experiments will shed light on the cellular mechanisms responsible for functional neural remodeling both after damage and during normal development.