CELL BIOLOGY OF THE SYNAPSE

大脑皮层钙调蛋白和主要的51,000 MR蛋白的功能 突触后密度(PSD)及其生理意义 功能，将通过两种方式进行检验。首先，针对这两种蛋白质的抗体 将通过显微操作注射到神经细胞中，并对 在正常情况下，将对这些信号进行电生理监测 在去极化条件下。第二，一个自旋标签将被贴在 和自旋标记的钙调蛋白重组成PSD 突触小体的制备耗尽了钙调蛋白。突触体的制备 会以各种方式去极化，并将ESR信号记录下来观察任何 突触后反应中钙调蛋白运动的变化。在……里面 另一个项目，将尝试从 美国黑寡妇蜘蛛，自从生化程序用于 意大利蜘蛛没有成功。这一尝试将以强者为基础 毒素与突触膜的特异性结合及毒素的去除 通过它的抗体从细胞膜上分离出来。

The function of calmodulin and of the major 51,000 Mr protein in cerebral cortex postsynaptic densities (PSD), and the physiological significance of these funtions, will be examined in two ways. First, antibodies to these two proteins will be injected into neuronal cells by micromanipulation, and the effects of these will be monitored electrophysiologically, under normal conditions and under depolarization conditions. Second, a spin-label will be attached to calmodulin, and the spin-labeled calmodulin reconstituted into PSDs of a synaptosome preparation depleted of calmodulin. The synaptosome preparation will be depolarized in various ways, and the ESR signals recorded to observe any change in the movement of the calmodulin during the post-synaptic response. In another project, an attempt will be made to purify alpha-latrotoxin from American black widow spiders, since the biochemical procedures used for the Italian spiders were unsuccessful. This attempt will be based on the strong specific binding of the toxin to synaptic membranes and the removal of the toxin from the membranes by antibodies to it.