METABOLISM OF CELLS IN MUSCLE AND NERVOUS SYSTEM

目的是帮助满足一个重要的需求，即了解 肌肉和神经中不同种类细胞的生化性质 系统大脑的功能取决于各种细胞的相互作用， 彼此之间的亲密关系。多种细胞类型的混合物， 事实上，功能状态因细胞而异， 这意味着宏观的化学方法是有限的 有用性 肌肉也是细胞的紧密混合物， 化学成分相邻的肌肉纤维也有很大的不同 在特定酶之间的比例上是50倍， 在肌肉疾病中会被夸大。因此，这里也宏观 研究不够。 为了解决这一问题，有一种分析方法， 几乎无限的灵敏度，这使得测量成为可能 几乎任何酶或代谢物在单个神经细胞体，在细胞核， 大的神经元，在其他类似大小的神经元结构中，以及在小的神经元结构中， 单个肌肉纤维的碎片 本项目的目的是利用这种分析能力， 研究肌肉和神经系统的学科。在某些情况下，单个神经元 或其一部分被检查;在其他情况下， 研究了在肌肉的情况下，该技术是这样的， 可以分析单个纤维中的许多酶、代谢物 辅因子

The objective is to help satisfy an important need, the need to know the biochemical nature of the different kinds of cells in muscle and nervous system. Brain function depends on the interplay of diverse kinds of cells, grandly entangled with each other. The admixture of the many cell types and the fact that the functional state differs from cell to cell, and from moment to moment, means that macroscopic chemical approaches are limited in usefulness. Muscle is also an intimate mixture of cells which differ greatly in chemical composition. Muscle fibers lying next to each other vary as much as 50-fold in the ratios between particular enzymes, and the differences can be exaggerated in muscle disease. Therefore, here also macroscopic studies are inadequate. To attack this situation, an analytical methodology is available which has virtually unlimited sensitivity and which makes it possible to measure nearly any enzyme or metabolite in single nerve cell bodies, in nuclei of large neurons, in other neuronal structures of similar size, and in small pieces of individual muscle fibers. It is the purpose of this project to exploit this analytical capability in the study of muscle and the nervous system. In some cases, single neurons or portions thereof are examined; in other cases, small groups of cells are studied. In the case of muscle, the technique is such that the same individual fibers can be analyzed for a great many enzymes, metabolites and cofactors.