ASPECTS OF INNER EAR IMMUNOLOGY

这是这个项目的目的，以回答有关内部的基本问题， 耳免疫：1）内耳是免疫优势部位吗？也就是说， 它在有效的传入和传出肢体的影响下运作， 免疫反应？ 2)内耳是否容易受到 免疫复合物 3)能产生抗耳蜗的特异性抗体吗 抗原可以进入内耳， 影响其功能？ 4)感音神经性听力障碍患者 丧失或眩晕表明体液或细胞介导的免疫应答 是针对内耳的吗 通过原发性KLH致敏， 外淋巴室、内淋巴囊和CSF的再激发， 并通过利用灵敏的放射免疫测定法检测抗KLH 内耳免疫反应的强度将是 测定 此外，通过使用耳蜗电描记术， 将监测免疫反应的生理效应。 灯光、 免疫荧光和电子显微镜将提供组织学 由于体液或细胞介导的反应导致的损伤的证据。 T和B细胞 然后采用过继转移实验来进一步鉴定 负责观察到的反应的细胞成分。 最后， 将对患有文音神经性听力损失或眩晕的患者进行研究， 存在抗耳蜗抗体或淋巴细胞胚细胞生成， 在其临床过程中，

It is the purpose of this project to answer basic questions regarding inner ear immunity: 1) Is the inner ear an immunopriviledged site; that is, does it operate under the influence of effective afferent and efferent limbs of the immune response? 2) Is the inner ear susceptible to the effects of immune complexes? 3) Can specific antibody raised against cochlear antigens be demonstrated to gain access to the inner ear and possibly affect its function? 4) Do patients suffering from sensorineural hearing losses or vertigo demonstrate a humoral or cell mediated immune response directed against the inner ear? Through primary KLH sensitizations and rechallenges of the perilymphatic compartment, endolymphatic sac and CSF, and by utilizing a sensitive radioimmunoassay for the detection of anti-KLH antibodies the magnitude of the inner ear's immune response will be determined. Also, through the use of electro-cochleography, the physiological effects of immune reactions will be monitored. Light, immunofluorescence, and electron microscopy will provide histological evidence of injury due to humoral or cell mediated responses. T and B cell adoptive transfer experiments will then be employed to further identify the cellular componente responsible for the observed response. Lastly, patients with wensorineural hearing loss or vertigo will be studied for the presence of anticochlear antibodies or lymphocyte blastogenesis to chochlear antigen during their clinical course.