ANDROGEN REGULATION OF NEUROMUSCULAR FUNCTION

这项建议研究的特点，个体发生和机制。 雄激素诱导的喉部增殖。我们的重点是肌肉及其 雄激素受体。实验方法包括氚胸苷 放射自显影术雄激素受体分析，电子显微镜，类固醇 放射自显影和组织培养。我们将导致核扩散 并对其时间参数进行了表征。 回应。不同细胞类型的有丝分裂(例如，成肌细胞， 成纤维细胞)将在光学和电子显微镜水平上进行检查。 将对雄激素诱导的增殖的个体发育进行研究和相关 青春期的性二型性和生殖生理。我们会 喉肌雄激素受体测定及受体分析 不同个体发育的男性和女性的亲和力和特异性 状态和对内分泌刺激的反应。两者之间的关系 成肌细胞和成纤维细胞中的雄激素受体和增殖 在体外进行了检查。我们研究的长期目标是发现 激素调节性分化的机制 生殖行为的神经效应器。我们认为，关注 运动神经元-肌肉单元将在这方面提供强大的优势 分析。这些研究的结果应该有助于揭示类固醇的调节作用 肌发生和再生以及内分泌并发症的研究 神经肌肉疾病。

This proposal examines the characteristics, ontogeny and mechanisms of androgen-induced laryngeal proliferation. Our focus is on muscle and its androgen receptors. Experimental approaches include tritiated thymidine autoradiography androgen receptor assays, electron microscopy, steroid autoradiography and tissue culture. We will induce proliferation in the larynx of juveniles and characterize the temporal parameters of the response. Mitogenesis in different cell types (e.g., myoblasts, fibroblasts) will be examined at the light and electron microscopic level. The ontogeny of androgen-induced proliferation will be studied and related to sexual dimorphism and reproductive physiology at puberty. We will measure androgen receptors in laryngeal muscle and analyze receptor affinity and specificity in males and females at different ontogenetic states and in response to endocrine stimulation. Relations between androgen receptors and proliferation in myoblasts and fibroblasts will be examined in vitro. The long term objective of our research is to uncover mechanisms whereby hormones regulate sexual differentiation of neuroeffectors for reproductive behavior. We believe that a focus on the motor neuron-muscle unit will provide powerful advantages in such analyses. Results of these studies should shed light on steroid modulation of myogenesis and regeneration and on endocrine complications of neuromuscular disease.