SPINAL REGULATION OF SOMATIC SENSATIONS

这项研究计划的目标是开始评估 脊髓背角(REXED椎板)的棘上下行调制 IV-VI)神经元。这项建议的独特之处在于它将涉及 完整清醒的猫的单个脊髓神经元的细胞外记录。 这将允许对系统的正常运行进行评估 不会混淆麻醉、去大脑或脊髓的影响 横切面。对背角神经元的下行抑制作用 已经证实，包括假定的产生止痛剂的作用 宽动态范围(WDR)神经元下行抑制。这是假设 然而，由于尚不清楚，其止痛机制仍有待商榷。 WDR神经元的生理作用及其下行能力 抑制也可以改变非伤害性活动。几行 有证据表明，在完好无损的动物中，下行抑制可能 阻断大范围动态反应的背角神经元的能力 距离配置文件。尽管没有明确地认识到 大动态范围神经元的生理作用。他们一直是中心 在许多研究中，评估了生理或心理上的 两种改变背角神经元活动的药物操作 以及产生行为止痛。因为他们潜在的 在我们理解精神分析症的神经生理学方面起着重要作用。 至关重要的是，我们必须清楚地认识到 完整动物的宽动态范围神经元反应特性。AS 因此，这项建议的具体目标如下：1) 确定强直性下行抑制是否阻断了 脊髓背角腰段神经元对宽动态范围的反应 生理状态正常，清醒，戒毒，行为幼稚 猫。2)确定中缝核是否存在下行抑制 Magnus参与了WDR配置文件的这种调制。3)确定是否 来自中缝大核的下行抑制也参与了 正常大鼠背角神经元自发活动的抑制 动物。4)评价5-羟色胺阻断对大鼠背角的影响 完整动物体内的神经元。

The objective of this research plan is to begin to evaluate the effects of descending supraspinal modulation on spinal dorsal horn (Rexed laminae IV-VI) neurons. This proposal is unique in that it will involve extracellular recordings from single spinal neurons in intact, awake cats. This will permit an evaluationof the systems as they normally function without confounding influences of anesthesia decerebration or spinal cord transection. Descending inhibitory effects on dorsal horn neurons have been demonstrated, including the assumed analgesic-producing effect of descending inhibition of wide dynamic range (WDR) neurons. This assumed mechanism of analgesia is open to question, however, because of the unknown physiologic role of WDR neurons, as well as the ability of descending inhibition to alter non-nociceptive activity as well. Several lines of evidence would suggest that in the intact animal, descending inhibition may block the ability of dorsal horn neurons to respond with a wide dynamic range profile. In spite of the lack of a clear appreciation of the physiologic role of wide dynamic range neurons. They have been the center of many studies which have evaluated the ability of either physiological or pharmacological manipulations to both alter dorsal horn neuronal activity as well as produce behavioral analgesia. Because of their potentially important role in our understanding of the neurophysiology of analygesia. it is essential that we have a clear appreciation of factors that regulate wide dynamic range neuron response properties in the intact animal. As such, this proposal has, as its specific aims, the following: 1) to determine if tonically active descending inhibition blocks the ability of spinal dorsal horn lumbar neurons to respond with a wid dynamic range profile in the physiologically intace, awake, drug free, behaviorally naive cat. 2) to determine if descending inhibition from the nucleus raphe magnus is involved in this modulation of WDR profiles. 3) to determine if descending inhibition from the nucleus raphe magnus also contributes to the depression of spontaneous activity of dorsal horn neurons in intact animals. 4) to evaluate the effect of serotonin blockage on dorsal horn neurons in the intact animal.