INHALATION ANESTHETIC EFFECTS ON THE SPINAL DORSAL HORN

吸入麻醉对脊髓背角的影响

• 批准号: 2608918
• 负责人: JERRY COLLINS
• 金额: $ 21.14万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-06-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2608918
• 关键词: anesthesia complication; anesthetics; cats; dorsal horn; drug screening /evaluation; electrophysiology; enflurane; excitatory aminoacid; halothane; inhalation anesthesia; isoflurane; laboratory rat; microelectrodes; neurons; neuropharmacology; neurotransmitter agonist; neurotransmitter antagonist; neurotransmitter receptor; neurotransmitters; nitrous oxide; sensory thresholds; stimulus /response

A long-term goal of our laboratory is to understand how general anesthetics alter sensory processing in the spinal cord. Recent work in our laboratory has revealed anesthetic related changes in the size of peripheral receptive fields and neuronal responses to non-noxious receptive field stimulation. The specific aim of this research project is to use those observed anesthetic effects as a tool to define what transmitter systems are involved in modulation of spinal sensory processing by selected general anesthetic agents. That information will provide a better understanding of the pharmacologic mechanisms by which general anesthetics alter nervous system function. Two separate protocols will be employed to answer the following 4 questions: 1.) What neurotransmitter systems contribute to the ability of halothane to depress receptive field size and neuronal response to receptive field brushing? 2.) What neurotransmitter systems contribute to enflurane's ability to depress receptive field size while at the same time enhancing neuronal response to brushing of the remaining receptive field? 3.) Does the new inhalation anesthetic desflurane differ from halothane and enflurane in its effects on spinal sensory processing? 4.) What neurotransmitter systems contribute to the ability of desflurane to alter spinal sensory processing? Questions 1, 2, and 4 will be answered by studying the effects of systemically and/or spinally administered neurotransmitter receptor agonists and antagonists on halothane, enflurane and desflurane induced alterations in spinal dorsal horn neuronal responses to non-noxious stimulation of peripheral receptive fields in acute rat preparations. Question 3 will be answered by examining the effects of desflurane on spinal dorsal horn neuronal responses to non-noxious and noxious receptive field stimulation in a chronic cat preparation. These studies should define, at the receptor level, systems that contribute to the ability of selected anesthetics to alter spinal sensory processing. While ultimate mechanisms of action are molecular in nature, results from this study will help to define neurotransmitter systems that are likely to be the mechanism by which molecular actions of anesthetics are translated into systemic effects that alter the ability of the nervous system to process sensory information.

我们实验室的一个长期目标是了解 麻醉剂改变了脊髓的感觉处理。最近的工作在 我们的实验室发现了与麻醉有关的大小变化 外周感受野和神经元对非伤害性反应 感受野刺激。这项研究项目的具体目的是 将这些观察到的麻醉效果作为一种工具来定义 递质系统参与脊髓感觉的调节 由选定的全身麻醉剂进行加工。这些信息将会 提供了更好的了解药理机制，通过 全身麻醉药会改变神经系统功能。 将使用两种不同的协议来回答以下4个问题 问题：1.)是什么神经递质系统对 氟烷抑制脑神经感受野大小和神经元反应 接受野刷新？2.)神经递质系统对 安氟醚同时抑制感受野大小的能力 增强神经元对刷除剩余感受野的反应？ 3.)新型吸入麻醉剂地氟烷与氟烷有区别吗？ 以及安氟醚对脊髓感觉加工的影响？什么 神经递质系统有助于地氟烷改变 脊椎感觉处理？ 问题1、2和4将通过研究以下因素的影响来回答 全身和/或脊椎给药的神经递质受体 氟烷、安氟醚和地氟烷的激动剂和拮抗剂 脊髓背角神经元对非伤害性反应的改变 急性大鼠准备时外周感受野的刺激。 问题3将通过检查地氟烷对 脊髓背角神经元对非伤害性和伤害性感受性的反应 慢性CAT准备中的场刺激。 这些研究应该在受体水平上定义 有助于选定麻醉剂改变脊椎感觉的能力 正在处理。虽然最终的作用机制本质上是分子的， 这项研究的结果将有助于定义神经递质系统 很可能是麻醉剂分子作用的机制 会转化为系统效应，从而改变神经系统的能力 处理感官信息的系统。