INHALATION ANESTHETIC EFFECTS ON THE SPINAL DORSAL HORN

吸入麻醉对脊髓背角的影响

• 批准号: 3304307
• 负责人: JERRY COLLINS
• 金额: $ 22.92万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-06-01 至 1995-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3304307
• 关键词: anesthesia complication; anesthetics; catheterization; cats; computer data analysis; dorsal column; electrophysiology; enflurane; halothane; inhalation anesthesia; isoflurane; neuropharmacology; nitrous oxide; sensory thresholds; spinal cord mapping

The goal of this research plan is to examine the effects of inhalation anesthetic agents on sensory processing in the spinal dorsal horn of physiologically intact, awake, drug-free animals. The proposal is unique in that it will employ extracellular recordings from neurons in intact, awake animals allowing evaluation of systems as they function normally without confounding influences of anesthesia, decerebration or spinal cord transection. We have recently demonstrated a profound effect of pentobarbital and propofol on spinal dorsal horn neurons but an apparent lack of similar effects by ketamine. An understanding of the mode of action of general anesthetics is an elusive but important goal. By documenting specific effects of anesthetic agents on sensory neurons in the spinal dorsal horn, we hope eventually to be able to define the pharmacology responsible for the observed changes- knowledge that may provide further insights into the mechanisms of action of general anesthetics. While recording extracellular activity from single spinal dorsal horn neurons in physiologically intact, awake, drug-free animals, we propose to determine baseline neuronal responses to noxious and non-noxious somatic stimuli induce anesthesia with the short-acting intravenous anesthetic propofol and maintain anesthesia with varying concentrations of halothane, enflurane, isoflurane and nitrous oxide. At a time when propofol effects have dissipated and an equilibrium state is established with the inhalation agent, we will evaluate the neuronal response to the same stimuli used in baseline studies. These results, when combined with data from intravenously administered anesthetics will help to define the effect of general anesthetics on sensory processing in spinal dorsal horn neurons and may provide insight into the mechanisms of general anesthesia.

这项研究计划的目标是检查吸入的影响 麻醉剂对脊髓背角感觉加工的影响 生理上完整的清醒的无毒动物提案是独一无二的 因为它将使用来自完整神经元的细胞外记录， 清醒的动物，允许在系统正常运行时对其进行评估 无麻醉、去大脑或脊髓的混杂影响 脊髓横断。 我们最近已经证明了一个深刻的影响， 戊巴比妥和异丙酚对脊髓背角神经元的作用，但明显 氯胺酮没有类似的效果。 对模式的理解 全身麻醉药的作用是一个难以捉摸但重要的目标。 通过 记录麻醉剂对感觉神经元的特定作用， 脊髓背角，我们希望最终能够定义 药理学负责观察到的变化-知识， 提供进一步的见解，一般的行动机制， 麻醉剂 同时记录单个脊髓背角的细胞外活动 神经元在生理完整，清醒，无药物的动物，我们建议 以确定神经元对有害和无害 躯体刺激诱发短效静脉麻醉 不同浓度异丙酚麻醉和维持麻醉 氟烷安氟醚异氟醚和一氧化二氮 之际 丙泊酚效应消失，并建立平衡状态 与吸入剂，我们将评估神经元的反应， 与基线研究中使用的刺激相同。 这些结果结合起来 静脉注射麻醉剂的数据将有助于 确定全身麻醉药对脊髓感觉处理的影响 背角神经元，并可能提供深入了解的机制 全麻