INHALATION ANESTHETIC EFFECTS ON THE SPINAL DORSAL HORN

吸入麻醉对脊髓背角的影响

• 批准号: 3304308
• 负责人: JERRY COLLINS
• 金额: $ 19.77万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-06-01 至 1995-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3304308
• 关键词: anesthesia complication; anesthetics; catheterization; cats; computer data analysis; dorsal column; electrophysiology; enflurane; halothane; inhalation anesthesia; isoflurane; neuropharmacology; nitrous oxide; sensory thresholds; spinal cord mapping

The goal of this research plan is to examine the effects of inhalation anesthetic agents on sensory processing in the spinal dorsal horn of physiologically intact, awake, drug-free animals. The proposal is unique in that it will employ extracellular recordings from neurons in intact, awake animals allowing evaluation of systems as they function normally without confounding influences of anesthesia, decerebration or spinal cord transection. We have recently demonstrated a profound effect of pentobarbital and propofol on spinal dorsal horn neurons but an apparent lack of similar effects by ketamine. An understanding of the mode of action of general anesthetics is an elusive but important goal. By documenting specific effects of anesthetic agents on sensory neurons in the spinal dorsal horn, we hope eventually to be able to define the pharmacology responsible for the observed changes- knowledge that may provide further insights into the mechanisms of action of general anesthetics. While recording extracellular activity from single spinal dorsal horn neurons in physiologically intact, awake, drug-free animals, we propose to determine baseline neuronal responses to noxious and non-noxious somatic stimuli induce anesthesia with the short-acting intravenous anesthetic propofol and maintain anesthesia with varying concentrations of halothane, enflurane, isoflurane and nitrous oxide. At a time when propofol effects have dissipated and an equilibrium state is established with the inhalation agent, we will evaluate the neuronal response to the same stimuli used in baseline studies. These results, when combined with data from intravenously administered anesthetics will help to define the effect of general anesthetics on sensory processing in spinal dorsal horn neurons and may provide insight into the mechanisms of general anesthesia.

这项研究计划的目标是检查吸入的影响。 麻醉剂对大鼠脊髓背角感觉加工的影响 生理上完好无损，清醒的，没有毒品的动物。这个提议是独一无二的 因为它将使用完整的神经元的细胞外记录， 唤醒的动物允许在系统正常工作时对其进行评估 不会混淆麻醉、去大脑或脊椎的影响 脐带横断。我们最近展示了一种深刻的影响 戊巴比妥和异丙酚对脊髓背角神经元的影响 氯胺酮没有类似的作用。对教育模式的一种理解 全身麻醉药的作用是一个难以实现但很重要的目标。通过 记录麻醉剂对大脑皮层感觉神经元的特殊影响 脊椎后角，我们希望最终能够定义 对观察到的变化负责的药理学-知识可能 提供对通用电气的作用机制的进一步见解 麻醉剂。 同时记录单个脊髓背角的细胞外活动 在生理完好、清醒、无药物的动物中，我们提出了 测定伤害性和非伤害性的基线神经元反应 短效静脉注射躯体刺激诱导麻醉 不同浓度异丙酚和维持性麻醉 氟烷、安氟醚、异氟烷和一氧化二氮。在这个时候， 异丙酚的作用已经消散，建立了平衡状态 使用吸入剂，我们将评估神经元对 与基线研究中使用的刺激相同。这些结果结合在一起， 来自静脉注射麻醉剂的数据将有助于 明确全身麻醉药对脊髓感觉加工的影响 背角神经元，并可能提供洞察的机制 全身麻醉。