SPINAL REGULATION OF SOMATIC SENSATIONS

本研究计划的目标是开始评估 脊髓背角（Rexed板）的下行脊髓上调制 IV-VI）神经元。 这项提案的独特之处在于，它将涉及 细胞外记录的单个脊髓神经元在完整的，清醒的猫。 这将允许对系统进行正常功能的评估 无麻醉去脑或脊髓的混杂影响 横切 对脊髓背角神经元的下行抑制作用 已被证明，包括假定的镇痛生产效果， 宽动态范围（WDR）神经元的下行抑制。 这假设 然而，镇痛机制是开放的问题，因为未知的 WDR神经元的生理作用，以及下降的能力， 抑制以改变非伤害感受活性。 几行 有证据表明，在完整的动物中，下行抑制可能 阻断背角神经元以宽动态响应的能力 距离剖面图 尽管缺乏明确的认识， 宽动态范围神经元的生理作用。 他们一直是 在许多研究中， 改变背角神经元活性的药理学操作 同时产生行为镇痛。 由于其潜在的 在我们理解分析记忆的神经生理学中起着重要作用。 我们必须清楚地认识到， 完整动物中的宽动态范围神经元响应特性。 作为 因此，这项建议的具体目标如下：1） 确定紧张性活性下行抑制是否阻断了 脊髓背角腰段神经元的反应具有广泛的动态范围 在生理学上，清醒，无药物，行为幼稚 目录 2)以确定是否来自中缝核的下行抑制 Magnus参与了WDR轮廓的这种调制。 3)以确定是否 来自中缝大核的下行抑制也有助于 抑制脊髓背角神经元的自发活动 动物 4)评价5-羟色胺阻断对脊髓背角的影响 完整动物的神经元。

The objective of this research plan is to begin to evaluate the effects of descending supraspinal modulation on spinal dorsal horn (Rexed laminae IV-VI) neurons. This proposal is unique in that it will involve extracellular recordings from single spinal neurons in intact, awake cats. This will permit an evaluationof the systems as they normally function without confounding influences of anesthesia decerebration or spinal cord transection. Descending inhibitory effects on dorsal horn neurons have been demonstrated, including the assumed analgesic-producing effect of descending inhibition of wide dynamic range (WDR) neurons. This assumed mechanism of analgesia is open to question, however, because of the unknown physiologic role of WDR neurons, as well as the ability of descending inhibition to alter non-nociceptive activity as well. Several lines of evidence would suggest that in the intact animal, descending inhibition may block the ability of dorsal horn neurons to respond with a wide dynamic range profile. In spite of the lack of a clear appreciation of the physiologic role of wide dynamic range neurons. They have been the center of many studies which have evaluated the ability of either physiological or pharmacological manipulations to both alter dorsal horn neuronal activity as well as produce behavioral analgesia. Because of their potentially important role in our understanding of the neurophysiology of analygesia. it is essential that we have a clear appreciation of factors that regulate wide dynamic range neuron response properties in the intact animal. As such, this proposal has, as its specific aims, the following: 1) to determine if tonically active descending inhibition blocks the ability of spinal dorsal horn lumbar neurons to respond with a wid dynamic range profile in the physiologically intace, awake, drug free, behaviorally naive cat. 2) to determine if descending inhibition from the nucleus raphe magnus is involved in this modulation of WDR profiles. 3) to determine if descending inhibition from the nucleus raphe magnus also contributes to the depression of spontaneous activity of dorsal horn neurons in intact animals. 4) to evaluate the effect of serotonin blockage on dorsal horn neurons in the intact animal.