MOLECULAR ORGANIZATION OF NEURON TERMINALS

脊椎动物的突触是一种特殊形式的细胞-细胞 对神经系统功能至关重要 等离子体 膜和下面的相关细胞质蛋白 突触的前突触侧和后突触侧都形成一个 高度专业化的，超微结构独特的复合体， 许多生物化学和生理学的细胞内定位 调节突触事件。 这项研究旨在检测 神经系统突触前末梢的组织。 这将 通过强大的组合， 记录的技术：标记突触前， 亚细胞胞质区室内的终端， 轴突运输，B）放射性标记的突触前 通过仔细和严格的制备突触体， 和c）使放射性标记的突触体经受 生物化学实验，旨在测试 放射性标记的蛋白质在细胞内的功能作用 terminals. 我们可能通过基础研究获得的信息 正常的生物化学、分子组织和功能 ”““这将有助于理解，“他说。 突触前末梢执行重要的生物学任务 在疾病状态的压力下，和B）在辨别 影响突触功能障碍的根源 直接.

The vertebrate synapse is a specialized form of cell-cell contact critical in nervous system function. The plasma membrane and underlying associated cytoplasmic proteins of both the pre-and post synaptic sides of the synapse form a highly specialized, ultrastructurally unique complex that is the intracellular location of many biochemically and physiologically regulated synaptic events. The proposed study is designed to examine the molecular organization of nervous system presynaptic terminals. This will be accomplished by the powerful combination of well- documented technologies: labelling of the presynaptic, subcellular cytoplasmic compartments within the terminal by axonal transport, b) the enrichment of radiolabeled presynaptic terminals by careful and rigorous preparation of synaptosomes, and c) the subjection of the radiolabeled synaptosomes to biochemical experiments that are designed to test the functional role of the radiolabeled proteins within the terminals. Information we may thus obtain through basic research on normal biochemistry, molecular organization, and functioning of the synapse will aid a) in understanding the way the presynaptic terminal performs its important biological tasks under the stress of disease states, and b) in discerning the origins and source of malfunctions that affect the synapse directly.