MOLECULAR CONTROL OF CNS GROWTH AND REGENERATION

这项研究的目的是阐明控制的分子机制。 中枢神经系统内轴突的生长和再生。第二信使系统 被假定的生长因子(S)激活的被分析等 纯化因子(S)。将使用的主要实验系统是神经。 从胎鼠脑和培养的大鼠脑组织中分离生长锥体片段 胎鼠大脑皮层。 在未来一年，我们会进行以下研究： 1)一种多肽因子的进一步纯化和鉴定 激活中枢神经系统生长锥体内多磷肌醇的裂解。 2)分析这种假定的生长因子对蛋白质的影响 生长锥体中的磷酸化。 3)该多肽因子参与肌醇磷脂的分析 代谢、钙离子和蛋白激酶C在生长激素调节中的作用 运动性，使用适当的药物局部应用于神经元 在培养和视频显微镜下萌发。 4)膜蛋白pp46的进一步表征 Ca~(2+)/钙调蛋白依赖的蛋白激酶C。

This research is aimed at elucidating the molecular mechanisms that control axonal growth and regeneration in the CNS. Second-messenger systems activated by putative growth factor(s) are to be analyzed and such factor(s) purified. The main experimental systems to be used are nerve growth cone fragments (GCPs) isolated from fetal rat brain and cultures of fetal rat cerebral cortex. In the coming year, the following studies will be performed: 1) The further purification and characterization of a peptide factor activating polyphosphoinositide cleavage in CNS growth cones. 2) Analysis of the effects of this putative growth factor on protein phosphorylation in the growth cone. 3) Analysis of the involvement of this peptide factor, of phosphoinositide metabolism, Ca2+, and protein kinase C in the regulation of growth cone motility, using localized application of appropriate agents to neurons sprouting in culture and video-microscopy. 4) Further characterization of membrane protien pp46, a major substrate of a Ca2+/calmodulin-dependent kinase and protein kinase C.