AIDS VIRUS INFECTION OF HUMAN NEURONAL CELLS

艾滋病病毒感染与多种疾病有关 神经综合征，从周围神经病到 痴呆症。尽管人类免疫缺陷病毒(HIV)已经 从小鼠脑组织和脑脊液中分离得到 感染患者，神经细胞病毒广泛传播的证据 还没有演示复制。 我们有初步证据表明，限制人类感染 神经母细胞瘤细胞存在于组织培养中。我们将测试 假设限制性(或不完全)HIV感染发生在 建立神经细胞来源的组织培养模型系统 用于研究艾滋病毒对人脑细胞的影响。 这些研究将测试病毒基因在 一系列表型不同的神经母细胞瘤细胞类型。我们 将评估细胞功能障碍，以 特定宿主细胞基因表达的改变，是由 感染艾滋病毒。 最后，我们将确定艾滋病毒感染是否会影响 每个神经母细胞瘤的分化能力，以及是否 细胞分化程度改变细胞分化程度 艾滋病毒感染的允许性。

AIDS virus infection has been associated with a variety of neurological syndromes ranging from peripheral neuropathy to dementia. Although the Human Immunodeficiency Virus (HIV) has been isolated from brain tissue and cerebro-spinal fluid of infected patients, evidence for wide spread nerve cell viral replication has not been demonstrated. We have preliminary evidence that restricted infection of human neuroblastoma cells occurs in tissue culture. We will test the hypothesis that restrictive (or incomplete) HIV infection occurs in cells of neuronal origin to develop a tissue culture model system for the study of HIV effects on human brain cells. These studies will test the extent of viral gene expression in a series of phenotypically different neuroblastoma cell types. We will assess whether cell dysfunction, measured in terms of alteration of specific host cell gene expression, is altered by infection with HIV. Finally, we will determine whether HIV infection affects the ability of each neuroblastoma to differentiate, and whether the level of cellular differentiation alters the degree of permissiveness of HIV infection.