VENTRAL MEDULLA OBLONGATA AND CARDIOVASCULAR CONTROL

腹侧延髓调节交感传出活动， 心血管系统。 含有P物质（SP）的神经元和 促甲状腺激素释放激素（TRH）从腹侧延髓投射到 脊髓中间外侧细胞柱（IML）（起源部位） 节前交感神经元）。 药理学实验表明， SP介导腹侧交感神经兴奋性反应 髓质 建议的实验旨在（1）进一步阐明 SP和TRH在延髓腹侧调节中的作用 通过研究TRH受体的细胞定位， 和交感心血管效应的IML TRH受体激活，通过 在体内评估腹侧延髓激活对指数的影响 的SP和TRH周转，并通过确定SP和TRH的影响， 类似物对局部脊髓和外周血流量的影响;以及（2） 提供更完整的信息，化学神经解剖学的 通过研究终末的起源和细胞定位， 和腹侧紧张性活动GABA能神经支配的受体 延髓SP和TRH神经元，并评估SP和TRH共存， 延髓腹侧的非肾上腺素能神经元和IML的终末。 我们将使用神经解剖学和神经化学技术（神经束 示踪，免疫细胞化学，受体放射自显影，肽周转， 放射免疫测定法）和体内神经药理学技术（CNS 注射和监测心率、血压和局部血液 流动）。 这些研究将提供有关化学神经解剖学的信息， 传出（SP，TRH）和传入（GABA）神经元系统的功能作用 腹侧髓质的一部分 这些信息对于理解 中枢神经系统控制心血管系统，并可能使新的设计， 治疗和/或预防心血管疾病的治疗方法和药物 疾病

The ventral medulla oblongata regulates sympathetic efferent activity to the cardiovascular system. Neurons containing substance P (SP) and thyrotropin releasing hormone (TRH) project from the ventral medulla to the spinal cord intermediolateral cell column (IML) (the site of origin of preganglionic sympathetic neurons). Pharmacologic experiments suggest that SP mediates sympathoexcitatory responses elicited from the ventral medulla. The proposed experiments are intended (1) to further elucidate the role of SP and TRH in the ventral medullary regulation of cardiovascular function by studying the cellular location of TRH receptors and sympathetic cardiovascular effects of IML TRH receptor activation, by assessing in vivo the effects of ventral medullary activation on an index of SP and TRH turnover, and by determining the effects of SP and TRH analogs on regional spinal cord and peripheral blood flow; and (2) to provide more complete information on the chemical neuroanatomy of the ventral medulla by studying the origin, and cellular location of terminals and receptors of the tonically active GABAergic innervation of ventral medullary SP and TRH neurons, and to evaluate SP and TRH coexistence in nonserotonergic neurons in the ventral medulla and terminals in the IML. We will use neuroanatomical and neurochemical techniques (neuronal tract tracing, immunocytochemistry, receptor autoradiography, peptide turnover, radioimmunoassay), and in vivo neuropharmacological techniques (CNS injections and monitering of heart rate, blood pressure, and regional blood flow). These studies will provide information on the chemical neuroanatomy and functional role of efferent (SP, TRH) and afferent (GABA) neuronal systems of the ventral medulla. This information is vital to the understanding of CNS control of the cardiovascular system and may enable the design of new therapeutic approaches and drugs to treat and/or prevent cardiovascular diseases.