VENTRAL MEDULLARY TRANSMITTERS & CARDIOVASCULAR CONTROL

位于延髓腹侧的神经元投射到胸侧 中间外侧细胞柱（IML）和调节交感神经活动， 心血管系统。 发现了许多假定的神经递质 单独或共存于IML投射腹侧的细胞体中 位于中缝中线和侧脑室的髓神经元 地区 P物质（SP）、5-羟色胺（5-HT）和促甲状腺素释放 激素（TRH）共存于单个IML投射细胞中，神经激肽A (NKA)，另一种与SP相关的速激肽肽，也可能在 这些神经元。 我们认为这些共定位的神经化学物质 药物在IML中释放并影响心血管系统， 直接通过与IML神经元的相互作用或间接通过修饰 另一种共定位剂的影响 为了验证这一点，我们将：（1） 获得关于这4个神经元的化学神经解剖学的更多信息 IML投射腹侧延髓中共存的假定递质系统 （2）确定哪种共定位的神经化学物质在其 （3）评估多个共域假定的 递质及其相互作用对心血管控制。 的 在这些研究中使用的技术包括：多重荧光 用免疫细胞化学和逆行示踪剂标记单细胞， 在体外受体放射自显影，在体外和体内的研究， 释放推定的递质，以及体内药理学和血液动力学 老鼠的研究 这些研究将提供有关神经化学和功能的信息。 在IML中存在多个假定的递质的意义- 投射神经元，并提供了一个可能的神经化学基础的见解 用于离散调制和最终的治疗操作， 交感神经输入到心血管系统。 此外该 这些信息将为神经生物学提供更全面的见解。 共同定位的神经化学物质

Neurons located in the ventral medulla project to the thoracic intermediolateral cell column (IML) and modulate sympathetic activity to the cardiovascular system. Numerous putative neurotransmitters are found either alone or coexisting in cell bodies of IML-projecting ventral medullary neurons that are located in the midline raphe and paraphyramidal region. Substance P (SP), serotonin (5HT), and thyrotropin-releasing hormone (TRH) coexist in individual IML-projecting cells, Neurokinin A (NKA), another tachykinin peptide related to SP, may also be colocalized in these neurons. We propose that each of these colocalized neurochemical agents is released in the IML and affects the cardiovascular system either directly through interactions with IML neurons or indirectly by modifying the effects of another colocalized agent. To test this, we will: (1) obtain additional information on the chemical neuroanatomy of these 4 coexisting putative transmitter systems in IML-projecting ventral medullary neurons; (2) determine which of the colocalized neurochemical on their release; (3) asses the influence of the multiple colocalized putative transmitters and their interactions on cardiovascular control. The techniques to be used in these studies include: multiple fluorescence labeling of single cells with immunocytochemistry and retrograde tracers, in vitro receptor autoradiography, both in vitro and in vivo studies of the release of putative transmitters, and in vivo pharmacologic and hemodynamic studies in rats. These studies will provide information on the neurochemical and functional significance of the presence of multiple putative transmitters in IML- projecting neurons and provide insights into a possible neurochemical basis for discrete modulation and ultimately therapeutic manipulation of sympathetic input to the cardiovascular system. In addition, the information will provide more general insights into the neurobiology of colocalized neurochemical.