VENTRAL MEDULLARY TRANSMITTERS & CARDIOVASCULAR CONTROL

位于延髓腹侧的神经元投射到胸腺 中间外侧细胞柱(IML)和调节交感神经活动 心血管系统。大量推测的神经递质被发现 单独或共存于IML投射的腹侧胞体 位于中缝中线和中缝旁的延髓神经元 区域。P物质(SP)、5-羟色胺(5HT)与促甲状腺激素释放 激素(TRH)共存于单个IML投射细胞，即神经激动素A 另一种与SP相关的速激肽(Nka)也可能共定位于 这些神经元。我们认为这些共同定位的神经化学物质中的每一个 药物在IML中释放，并影响心血管系统 直接通过与IML神经元的交互或通过修改 另一种共定位因子的影响。为了测试这一点，我们将：(1) 获取有关这4种化合物的化学神经解剖学的更多信息 延髓腹侧IML投射的共存递质系统 神经元；(2)确定哪些共定位的神经化学物质在其 释放；(3)评估多个共定位假设的影响 递质及其在心血管控制中的相互作用。这个 在这些研究中使用的技术包括：多重荧光 用免疫细胞化学和逆行示踪剂标记单细胞 体外受体放射自显影，体外和体内研究 推定递质的释放以及体内药理学和血流动力学 在老鼠身上进行的研究。 这些研究将提供有关神经化学和功能的信息。 在IML中存在多个假定的发射器的意义- 投射神经元并提供对可能的神经化学基础的见解 用于离散调制和最终的治疗性操作 交感神经对心血管系统的输入。此外， 信息将提供对神经生物学的更全面的见解 共同定位的神经化学物质。

Neurons located in the ventral medulla project to the thoracic intermediolateral cell column (IML) and modulate sympathetic activity to the cardiovascular system. Numerous putative neurotransmitters are found either alone or coexisting in cell bodies of IML-projecting ventral medullary neurons that are located in the midline raphe and paraphyramidal region. Substance P (SP), serotonin (5HT), and thyrotropin-releasing hormone (TRH) coexist in individual IML-projecting cells, Neurokinin A (NKA), another tachykinin peptide related to SP, may also be colocalized in these neurons. We propose that each of these colocalized neurochemical agents is released in the IML and affects the cardiovascular system either directly through interactions with IML neurons or indirectly by modifying the effects of another colocalized agent. To test this, we will: (1) obtain additional information on the chemical neuroanatomy of these 4 coexisting putative transmitter systems in IML-projecting ventral medullary neurons; (2) determine which of the colocalized neurochemical on their release; (3) asses the influence of the multiple colocalized putative transmitters and their interactions on cardiovascular control. The techniques to be used in these studies include: multiple fluorescence labeling of single cells with immunocytochemistry and retrograde tracers, in vitro receptor autoradiography, both in vitro and in vivo studies of the release of putative transmitters, and in vivo pharmacologic and hemodynamic studies in rats. These studies will provide information on the neurochemical and functional significance of the presence of multiple putative transmitters in IML- projecting neurons and provide insights into a possible neurochemical basis for discrete modulation and ultimately therapeutic manipulation of sympathetic input to the cardiovascular system. In addition, the information will provide more general insights into the neurobiology of colocalized neurochemical.