DETERMINATION OF SELENIUM IN CARDIAC BIOPSY SAMPLES

这项原子光谱学研究的远程目标是开发 并改进了金属和金属的定量分析方法 生物样品中的非金属元素需要微量分析或 痕迹分析。这个项目的具体目标是开发一种 一种足够灵敏和准确的分析方法 研究硒在扩张型心肌病中的作用，并进行 斯特恩博士收集的心脏活检样本的实际分析 大约两年的时间。这将涉及以下步骤： 开发一种灵敏、准确和可重复性的分析方法 1毫克活检组织样本中的硒含量。石墨炉原子 最近发展起来的吸收光谱(GFAAS)方法 将对重量为8至10毫克的组织样本进行硒的测定 进一步微型化，以分析心脏的毫升-毫克样品 经导管取活组织使硒在体内的作用 可以对心肌病进行评估。 B.通过分析国家统计局标准样品(国家统计局1577和1571)来验证方法。 以及其他可用的样品，如牛心。进行同质性研究 在心肌样本上，以确定是否可能进行 对毫克大小的组织样品进行硒分析并产生可靠的结果 结果：初步实验将使用牛肉He 艺术。 C.分析心脏组织的实际活组织样本，旨在 确定血清硒是否是心脏硒的指标。一项研究 将进行包括分析心脏活检组织的 硒的样品和随后的统计评估 结果。每月五到十个活组织检查样本，用于 组织学评估，将在组织学检查后进行硒分析 研究已经完成。拟议的研究将由斯特恩博士提交给 田纳西大学(孟菲斯)的适当委员会 批准。所需的分析将在孟菲斯州立大学根据 威廉姆斯博士的监督。 以前在人类和动物组织中定量测定硒的工作是 对重量为一克或更多的样品进行测试，通常从 身体。活检样本必须限制在大约1毫克以内。 来自如此小样本的可靠、可重现的数据将使医疗 专家评估了硒在扩张型心肌病中的作用范围。 这项研究将提供一种灵敏、准确的检测技术 心脏活检组织和/或血清中的总硒。因此，该方法 然后将开发的样品用于分析5到10个心脏活检组织样本 每个月在两年的时间内，因此产生了相对较大的池 这些数据将被用来帮助解释硒在心脏中的作用 移植和扩张型心肌病。

The long range goals of this research in atomic spectroscopy are to develop and improve quantitative methods for the analysis of both metal and nonmetal elements in biological samples requiring either microanalysis or trace analysis. The specific objectives of this project are to develop an analytical method which is sufficiently sensitive and accurate to support a study of the role of selenium in dilated cardiomyopathy, and to conduct the actual analyses of heart biopsy samples collected by Dr. Stern over approximately a 2-year period. This will involve the following steps: a. Develop a sensitive, accurate, and reproducible method for the analysis of selenium in 1-mg samples of biopsy tissue. The graphite furnace atomic absorption spectroscopic (GFAAS) method recently developed for the determination of selenium in tissue samples weighing 8 to 10 mg will be further miniaturized in order to analyze the ml-mg samples of cardiac biopsy tissue taken by hear catheterization so that the role of selenium in cardiomyopathy can be assessed. b. Verify the method by analyzing NBS standard samples (NBS 1577 and 1571), and other available samples such as beef heart. Conduct a homogeneity study on myocardium samples in order to determine if it is possible to perform selenium analyses on milligram size tissue samples and produce reliable results; the initial experiments will be conducted using beef he art. c. Analyze actual biopsy samples of cardiac tissue in a study designed to determine if serum selenium is an indicator for cardiac selenium. A study will be conducted which will involve the analysis cardiac biopsy tissue samples for selenium and the subsequent statistical evaluation of the results. Five to ten biopsy samples per month, which are taken for histological evaluation, will be analyzed for selenium after the histology studies are complete. The proposed study will be submitted by Dr. Stern to the appropriate committee at The University of Tennessee (Memphis) for approval. The required analyses will be done at Memphis State under the supervision of Dr. Williams. Previous work on quantitating selenium in human and animal tissues has been done on samples weighing a gram or more, and usually obtained from cadavers. Biopsy samples will necessarily be limited to approximately I mg. Reliable, reproducible data from such small samples will enable medical experts to assess the scope of selenium's role in dilated cardiomyopathy. This research will provide a sensitive, accurate technique for determining total selenium in cardiac biopsy tissue and/or blood serum. The method thus developed will then be used to analyze 5 to 10 heart biopsy tissue samples per month over a 2-year period thus producing a relatively large pool of data which will be used to help explain the role of selenium in heart transplants and dilated cardiomyopathy.