SkyScan 1172 High-resolution desk-top micro-CT system

SkyScan 1172 高分辨率台式显微 CT 系统

• 批准号: 7213201
• 负责人: JAMES C WILLIAMS
• 金额: $ 29.66万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-15 至 2008-03-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7213201
• 关键词: Antineoplastic Agents; Architecture; Area; Arts; Biological; Biopsy; Blood Vessels; Bone Development; Bone Diseases; Calcium; Calculi; Chronic Kidney Failure; Communities; Computer software; Dental caries; Deposition; Disease; Early Diagnosis; Felis catus; Funding; Future; Genetic; Hour; Image; Imagery; Kidney; Kidney Calculi; Laboratories; Limb structure; Lithotripsy; Maps; Myocardial Infarction; Operative Surgical Procedures; Organ; Osteolytic; Osteoporosis; Patients; Peripheral Vascular Diseases; Research; Resolution; Sampling; Scanning; Services; Shock; Slice; Specimen; Staging; Structure; System; Technology; Vascular calcification; Work; Wound Healing; X-Ray Computed Tomography; base; bone; reconstruction; scaffold; size; soft tissue; vascular bed

DESCRIPTION (provided by applicant): This proposal is for the purchase of a SkyScan 1172 micro computed tomography (CT) system, which will serve ten NIH-funded research groups. Our present micro CT system has been working at maximum capacity for five years, but its quality of image resolution is not adequate for applications to soft tissue or to any structures smaller than about 25 ¿m. The demand for micro CT on our campus continues to grow, and the proposed system will allow excellent resolution of fine structure even in low contrast samples, so that new applications for micro CT can be accommodated or developed. The SkyScan 1172 can image specimens with very high resolution-less than 1 ¿m voxel size and low-contrast resolution of 5 ¿m. A typical scan takes only about one hour, and the system comes with a reconstruction workstation of clustered PC's and special software to allow rapid calculation of image slices, so that a user can reconstruct a scan while the next specimen is scanning. This will provide the high throughput required to service the needs of our research community for micro CT. Applications for the proposed facility include: 1) Elucidation of the fine structure of kidney stones, for studies of the mechanisms of stone breakage by shock wave lithotripsy and study of stone formation. 2) Visualization of fine structure of kidney biopsies taken from stone patients during surgery, showing the earliest stages of the formation of kidney stones. 3) Detection of the earliest stages of dental caries. 4) Mapping and quantitation of vascular calcification in chronic renal disease. 5) Quantitation and imaging of vascular beds in limbs and organs injected with contrast fill, to study peripheral vascular disease and myocardial infarction. 6) Imaging and quantitation of bone structure in studies of osteoporosis, of the genetic basis of bone architecture, of osteolytic cancer, drug effects on bone, development of microcracks, wound healing in bone, and of bone regrowth in artificial scaffolds. The acquisition of the SkyScan 1172 is essential for continued progress on these and future projects requiring this state-of-the-art technology. Relevance. This proposal is for purchase of a new micro CT ("cat" scanner) system, which will be used to study biological specimens from several laboratories. This technology will make possible new discoveries in the areas of bone disease (such as osteoporosis), blood vessel disease (including the deposition of calcium in vessels in chronic kidney disease), tooth decay, and kidney stone disease.

描述（由申请人提供）：该提案是为了购买SkyScan 1172微型计算机断层扫描（CT）系统，该系统将为10个NIH资助的研究小组提供服务。我们目前的微型CT系统已经在最大容量下工作了五年，但其图像分辨率的质量不足以应用于软组织或小于约25 μ m的任何结构。我们校园对微型CT的需求持续增长，所提出的系统将允许即使在低对比度样品中也能实现精细结构的出色分辨率，从而可以容纳或开发微型CT的新应用。SkyScan 1172可以对样本进行高分辨率成像-体素尺寸小于1 μ m，低对比度分辨率为5 μ m。一次典型的扫描只需大约一个小时，系统配备了一个集群PC的重建工作站和特殊软件，可以快速计算图像切片，这样用户就可以在扫描下一个标本时重建扫描。这将提供所需的高吞吐量，以满足我们的研究社区对微型CT的需求。该设备的应用包括：1）阐明肾结石的精细结构，用于研究冲击波碎石术的碎石机制和研究结石形成。2)手术期间从结石患者身上取下的肾活检组织的精细结构的可视化，显示肾结石形成的最早阶段。3)检测龋齿的最早阶段。4)慢性肾脏疾病血管钙化的定位和定量。5)对注射造影剂的四肢和器官中的血管床进行定量和成像，以研究外周血管疾病和心肌梗死。6)骨质疏松症研究中骨结构的成像和定量，骨结构的遗传基础，溶骨性癌症，药物对骨的影响，微裂纹的发展，骨中的伤口愈合，以及人工支架中的骨再生。收购SkyScan 1172对于这些项目以及未来需要这种最先进技术的项目的持续进展至关重要。 本案无关这项建议是购买一个新的微型CT（"猫"扫描仪）系统，将用于研究来自几个实验室的生物标本。这项技术将可能在骨骼疾病（如骨质疏松症）、血管疾病（包括慢性肾脏疾病中血管中的钙沉积）、蛀牙和肾结石疾病等领域取得新的发现。

项目成果

Cortical and trabecular bone benefits of mechanical loading are maintained long term in mice independent of ovariectomy.

• DOI: 10.1002/jbmr.2143
• 发表时间: 2014
• 期刊: JOURNAL OF BONE AND MINERAL RESEARCH
• 影响因子: 6.2
• 作者: Warden, Stuart J.;Galley, Matthew R.;Hurd, Andrea L.;Richard, Jeffrey S.;George, Lydia A.;Guildenbecher, Elizabeth A.;Barker, Rick G.;Fuchs, Robyn K.
• 通讯作者: Fuchs, Robyn K.

Re: Grases et al.: Origin and types of calcium oxalate monohydrate papillary renal calculi (Urology 2010;76:1339-1345).

回复：Grases 等人：一水草酸钙乳头状肾结石的起源和类型（Urology 2010；76：1339-1345）。

• DOI: 10.1016/j.urology.2011.05.016
• 发表时间: 2011
• 期刊: Urology
• 影响因子: 2.1
• 作者: WilliamsJr,JamesC;Evan,AndrewP;Lingeman,JamesE;Evan,AndrewP
• 通讯作者: Evan,AndrewP

Compromised osseous healing of dental extraction sites in zoledronic acid-treated dogs.

• DOI: 10.1007/s00198-010-1268-4
• 发表时间: 2011-02
• 期刊: OSTEOPOROSIS INTERNATIONAL
• 影响因子: 4
• 作者: Allen, M. R.;Kubek, D. J.;Burr, D. B.;Ruggiero, S. L.;Chu, T. -M. G.
• 通讯作者: Chu, T. -M. G.

Reduced gravitational loading does not account for the skeletal effect of botulinum toxin-induced muscle inhibition suggesting a direct effect of muscle on bone.

重力负荷的减少并不能解释肉毒杆菌毒素诱导的肌肉抑制对骨骼的影响，表明肌肉对骨骼的直接影响。

• DOI: 10.1016/j.bone.2013.01.043
• 发表时间: 2013
• 期刊: Bone
• 影响因子: 4.1
• 作者: Warden,StuartJ;Galley,MatthewR;Richard,JeffreyS;George,LydiaA;Dirks,RachelC;Guildenbecher,ElizabethA;Judd,AshleyM;Robling,AlexanderG;Fuchs,RobynK
• 通讯作者: Fuchs,RobynK

Defective cancellous bone structure and abnormal response to PTH in cortical bone of mice lacking Cx43 cytoplasmic C-terminus domain.

缺乏 Cx43 胞质 C 端结构域的小鼠皮质骨松质骨结构缺陷和对 PTH 的异常反应。

• DOI: 10.1016/j.bone.2015.09.011
• 发表时间: 2015
• 期刊: Bone
• 影响因子: 4.1
• 作者: Pacheco-Costa,Rafael;Davis,HannahM;Sorenson,Chad;Hon,MaryC;Hassan,Iraj;Reginato,RejaneD;Allen,MatthewR;Bellido,Teresita;Plotkin,LilianI
• 通讯作者: Plotkin,LilianI