ANTINEOPLASTIC AGENTS FROM BLUE GREEN ALGAE

我们已经从两个加勒比海地区分离出两个系列的新型天然产品 蓝藻、水华鱼腥藻和蓝藻。全 这些次级代谢物中有一种在体外表现出良好的细胞毒性，其中一种 我们毫不含糊地指定了一种新的苯基色酮结构， 表现出极好的细胞毒性，ID50值在纳克范围内。 此外，后一种天然产物选择性地抑制RNA的合成。 两个癌细胞系。我们随后收集的这两个相同的 来自不同栖息地和季节的蓝绿色指出了 这些生命形式的极端化学变异性。这些植物的提取物 后者的收藏品具有很强的生物活性，但包含非常 薄层层析法测定的化学成分与以往的样品不同。 基于上述观察结果，我们建议对 这些和其他加勒比海蓝藻都有新的细胞毒素。此外，我们 计划探索环境因素对蓝藻的影响 次生代谢由季节性采集和下唇培养工作完成。 我们的长期目标是发现新的化疗药物 治疗肿瘤疾病，因此，为了进一步了解 细胞毒性的结构要求。

We have isolated two series of novel natural products from two Caribbean blue-green algae, Anabaina oscillarioides and Microcoleus lyngbyaceus. All of these secondary metabolites show good in vitro cytotoxicity and one, for which we have unambiguously assigned a novel styrylchromone structure, shows excellent cytotoxicity with ID50 values in the nanogram range. Further, this latter natural product selectively inhibits RNA synthesis in two cancerous cell lines. Our subsequent collections of these same two blue-greens from different habitats and seasons have pointed out the extreme chemical variability of these life forms. The extracts of these latter collections are strongly biologically active yet contain very different chemistry by TLC analysis than the previous collections. The above observations lead us to propose a systematic investigation of these and other Caribbean blue green algae for new cytotoxins. Further, we plan to explore the influence of environmental factors on blue green algal secondary metabolism by seasonal collections and labaoratory culture work. Our long term objectives are to discover new chemotherapeutic agents for treating neoplastic diseases, and hence, to further understand the structural requirements for cellular toxicity.