IMMUNE FUNCTIONS OF THE MACROPHAGE MAN/GLCNAC RECEPTOR

小鼠巨噬细胞Man/GlcNAc受体具有多种重要免疫功能 功能。为了了解该酶的功能和生化特性 关于受体，建议如下：(1)检查 免疫复合体对Man/GlcNAc受体功能的影响固定化络合物 例如DNP-抗DNP抗体或抗绵羊血清红细胞-绵羊单抗 红细胞幽灵将被用来调节巨噬细胞的受体 对酵母多糖的吞噬作用进行了研究。可溶性络合物，如TNP-牛 血清白蛋白-抗TNP抗体将用于竞争Man/GlcNAc 受体介导的辣根过氧化物酶摄取；(2)衍生 转染骨髓细胞的MAN/GlcNAc受体阳性细胞系 肺泡巨噬细胞和腹膜巨噬细胞表达SV4O或DNA； 用(A)法鉴定和鉴定Man/GlcNAc受体 免疫沉淀和聚丙烯酰胺凝胶电泳法分析 抗小鼠巨噬细胞异源抗血清制备巨噬细胞表面蛋白 被Man/GlcNAc受体阴性细胞系广泛吸收；(B) 大鼠X-大鼠融合单抗的研制及抗体筛选 抗小鼠受体活性。[125I]-抗鼠二抗-标记 杂交瘤上清液处理的巨噬细胞，以及阻断 杂交瘤细胞摄取酵母多糖和辣根过氧化物酶的研究 上清液将用于筛选；(C)亲和标记 甘露糖或辣根标记的MAN/GlcNAc受体 过氧化物酶低聚糖；和(4)功能作用的检测 MAN/GlcNAc受体指示巨噬细胞分泌H202和 巨噬细胞与肿瘤细胞的结合。低聚糖结合到 将使用聚合物、珠子或底物来触发巨噬细胞 分泌细胞糖蛋白、糖脂和低聚糖 从糖偶联物中释放出来的将在溶液中使用或偶联到 竞争肿瘤细胞与巨噬细胞结合的底物。这些 实验将进一步阐明碳水化合物在免疫中的作用 Man/GlcNAc受体在巨噬细胞功能中的功能和作用 (MB)

The mouse macrophage Man/GlcNAc receptor serves many important immune functions. To understand the function and biochemical properties of this receptor, the following is proposed: (1) examination of the effects of immune complexes on Man/GlcNAc receptor function. Immobilized complexes such as DNP-anti DNP antibody or monoclonal anti-sheep serythrocyte-sheep erythrocyte ghosts will be used to modulate macrophage receptors of the phagocytosis of zymosan studied. Soluble complexes such as TNP-bovine serum albumin-anti-TNP antibody will be used to compete for Man/GLcNAc receptor-mediated horseradish peroxidase uptake; (2) derivation of Man/GlcNAc receptor-positive cell lines by transfecting bone marrow cells and alveolar and peritoneal macrophages with SV4O Ori- DNA; (3) identification and characterization of the Man/GlcNAc receptor by (a) immunoprecipitation and polyacrylamide gel electrophoretic analysis of macrophage surface proteins by an antimouse macrophage heteroantiserum extensively absorbed with Man/GlcNAc receptor-negative cell line; (b) development of monoclonal antibodies by rat X rat fusions and screening for anti-mouse receptor activity. [125I]-anti-rat second antibody-labeling of hybridoma supernatant-treated macrophages, as well as blockage of macrophage uptake of zymosan and horseradish peroxidase by hybridoma supernatants will be used for screening; (c) affinity-labeling of the Man/GlcNAc receptor by labeled conjugates of mannose or horseradish peroxidase oligosaccharides; and (4) examination of the functional role of the Man/GlcNAc receptor in indicating macrophage secretion of H202 and macrophage-binding to tumor cells. Oligosaccharides conjugated to polymers, beads or substrates will be used for the triggering of macrophage secretion while cellular glycoprotein, glycolipids and oligosaccharides released from the glycoconjugates will be used in solution or coupled to substrates to compete for binding of tumor cells to macrophages. These experiments will further clarify the role of carbohydrates in immune functions and the role of the Man/GlcNAc receptor in macrophage functions. (MB)