ORGAN TRANSPLANTS: ANALYSIS OF MIGRATING LYMPHOCYTES

存在许多潜在的移植物排斥反应机制。对这些问题的研究 机制必须考虑致敏细胞的迁移模式； 在动物体内，移植物同种异体抗原可能远离淋巴库。 而特定的细胞只有在能够到达移植物的情况下才能发挥作用。 关于淋巴细胞贩运的调查结果尚不确定 贩运是特定的还是非特定的现象。问题所在 然而，这些先前的研究包括：(A)假设只有 激活的细胞是那些快速分裂的细胞，即那些占据 放射性标签。(B)标签对细胞能力的影响 迁移。(C)在下列情况下将标记细胞注入血管间室 事实上，他们通常可能无法访问。这些研究表明， 淋巴细胞迁移取决于(1)淋巴细胞迁移到的亚群 淋巴细胞属于，(2)淋巴细胞的激活状态，和(3) 宿主的免疫状态。 我们建议利用有限稀释分析技术来研究 致敏增殖性和细胞毒前期/细胞毒细胞的分布 通过同种异体海绵移植致敏小鼠。使用这些功能 对淋巴细胞的评估而不是放射性标记消除了 将放射性标记的细胞引入血管内间隙。的用法 小鼠的同种异体海绵移植模型允许轻松访问所有 包括渗入同种异体移植物的细胞的间隔室。

Many potential mechanisms of graft rejection exist. Study of these mechanisms must take into account migratory patterns of sensitized cells; in the animal the graft alloantigen may be far removed from lymphoid depots and a particular cell can only exert an effect if it can get to the graft. Investigators of lymphocyte trafficking have had uncertain results as to whether trafficking is a specific or nonspecific phenomenon. The problems with these previous studies however include: (a) An assumption that only activated cells are those rapidly dividing i.e. those which take up radioactive label. (b) The effect of the label on the ability of cells to migrate. (c) Injection of labeled cells into the vascular compartment when in fact they may not normally have access. These studies have shown that lymphocyte migration depends on (1) the subpopulation to which the lymphocyte belongs, (2) the state of activation of the lymphocyte, and (3) the host immune state. We propose to utilize the limiting dilution assay technique to study the distribution of sensitized proliferating and precytotoxic/cytotoxic cells throughout the sponge allograft sensitized mouse. Using these functional assessments of lymphocytes rather than radiolabel eliminates the need to introduce radiolabeled cells into the intravascular space. The use of the sponge allograft model in the mouse allows for easy access to all compartments including the cells infiltrating the allograft.