MODIFICATION OF LOCAL AND SYSTEMIC ALLOGRAFT REJECTION

局部和全身同种异体移植排斥的改变

• 批准号: 3142690
• 负责人: Nancy Ascher
• 金额: $ 16.64万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-30 至 1993-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3142690
• 关键词: antigen presenting cell; cell sorting; cyclosporines; delayed hypersensitivity; genetic strain; helper T lymphocyte; histocompatibility; histocompatibility antigens; immunosuppression; laboratory mouse; liver transplantation; pancreatic islet transplantation; suppressor T lymphocyte; tissue donors

A number of different mechanisms exist to effect allograft rejection. The role of the lymphocyte is central in graft rejection; ample evidence exists that the helper T cell and the cytolytic T cell can effect graft loss. It is also clear that events occurring locally at the graft site are distinct from the systemic response to the allograft. The sponge matrix allograft model provides ready access to cells which accumulate at the site of allograft rejection. In combination with limiting dilution analysis which can identify small numbers of sensitized cells, we can readily assess events associated with local and systemic allograft immunity. Using these functional assessments of lymphocytes rather than radiolabel eliminates the need to introduce radiolabelled cells into the intravascular space. We plan to use these techniques to define these local and systemic events according to the tissue transplanted. Once these events are defined for various tissue we plan to use either local or systemic immunosuppression to modify them. The long term goal is to employ local immunosuppression to render the animal's systemic immune apparatus intact.

存在许多不同的机制来影响同种异体移植 排斥反应 淋巴细胞在移植物中的作用是中心的 排斥反应;有充分的证据表明，辅助性T细胞和 细胞溶解性T细胞可影响移植物丢失。 并明确 在移植部位局部发生的事件不同于 对同种异体移植物的全身反应。 海绵基质同种异体移植模型提供了容易接近细胞的途径 在同种异体移植排斥反应部位聚集。 在 结合有限稀释分析， 少量的致敏细胞，我们可以很容易地评估事件 与局部和全身同种异体移植免疫相关。 使用这些 淋巴细胞功能评估，而不是放射性标记 消除了将放射性标记的细胞引入到 血管内空间 我们计划使用这些技术来定义 这些局部和全身性事件根据组织 移植的 一旦为各种组织定义了这些事件， 计划使用局部或全身免疫抑制来改变 他们 长期目标是采用局部免疫抑制， 使动物的全身免疫系统完好无损。