Y CHROMOSOME EXPRESSION AND EVOLUTION

本研究的目的是阐明Y病毒感染的机制。 染色体决定性别表型。为此，我们制定了一项 一种特异克隆Y染色体DNA的新方法并构建了 足够大以包含整个Y补码的库 染色体DNA序列。该库将用于分析Y编码 发育中的胚胎中的核糖核酸。这门课的学习有可能 确定Y染色体上启动 性别分化的发育途径。使用此库 我们还将研究DNA序列的保守性和漂移性 在脊椎动物两亿年的进化过程中。许多 患有性别分化发育障碍的儿童 携带涉及Y染色体的遗传异常。克隆的Y染色体 DNA将被用于建立染色体之间的关系 性分化异常、基因表达和异常模式 在人类身上。

The objective of this study is to elucidate the mechanism by which the Y chromosome determines sexual phenotype. To this end, we have devised a novel method for specifically cloning Ychromosome DNA and have constructed a library that is sufficiently large to contain the entire complement of Y chromosome DNA sequences. This library will be used to analyze Y encoded nRNAs in developing embryos. This class of study has the potential to identify transcribed genes on the Y chromosome which initiate the developmental pathway of sexual differentiation. Using this library of cloned Y DNA we will also study the conservation and drift of DNA sequences on the Y chromosome during 200 million years of vertebrate evolution. Many children suffering from developmental disorders of sexual differentiation carry genetic anomalies involving the Y chromosome. Cloned Y chromosome DNA will be used to establish the relationship between chromosome anomalies, gene expression and abnormal patterns of sexual differentiation in man.