VIP RECEPTOR IN THE RETINAL PIGMENT EPITHELIUM

视网膜色素上皮（RPE）是单层的视网膜色素上皮。 在脉络膜之间并列的专门的神经上皮细胞 和视网膜。 几种激素和神经递质存在于 发现脉络膜（11 - 14）或视网膜（15，16）中的突起会抬高 鸡RPE细胞内cAMP浓度 胚胎 本研究建议审查的可能性， 脉络膜的激素和神经递质 视网膜起源可作为RPE功能的调节剂。 在RPE的环AMP池的调节剂中， 血管活性肠肽（VIP）是最有效的一种。 的 因此，RPE上的VIP受体将被用作模型， 确定激素所起的作用， 神经递质调节RPE的功能。 拟议的研究将表征VIP受体在 以下方面与建立生理作用有关， RPE细胞中的这种受体： 1)VIP受体在顶和/或 基底外侧膜， 2)受体的功能研究：VIP对 电解质和大分子的传输; 3） VIP对RPE细胞的作用：腺苷酸刺激以外 细胞蛋白的环化酶和磷酸化，4） 视网膜色素上皮细胞上VIP受体的可视化 用放射自显影法检查眼睛。 目前提出的研究代表了一种努力，旨在 理解存在于 脉络膜RPE和视网膜，以及导致 视网膜脱离的症状有哪些？

The retinal pigment epithelium (RPE) is a single layer of specialized neuro-epithelial cells juxtaposed between the choroid and the retina of the eye. Several hormones and neurotransmitters existing either in the choroid (11-14) or in the retina (15, 16) are found to elevate the intracellular cyclic AMP concentration of the RPE from the chick embryos. The present study propose to examine the possibility that the hormones and neurotransmitters of the choroidal and/or retinal origin may function as modulators of the RPE functions. Among the modulators of the cyclic AMP pools of the RPE, the vasocative intestinal peptide (VIP) is the most effective one. The VIP receptor on the RPE is therefore to be used as a model in identifying the roles played by the hormones and neurotransmitters in modulating the functions of the RPE. The proposed study will characterize the VIP receptor in the following aspects relevant to establishing a physiological role for this receptor in the RPE cells: 1) Localization of the VIP receptors on the apical and/or the basolateral membrane, 2) Functional studie of the receptors: the effect of VIP on transport of electrolyte and macromolecule, 3) The mechanism of VIP action of the RPE cells: beyond stimulation of the adenylate cyclase and phosphorylation of the cellular proteins, 4) Visualization of the VIP receptors on the RPE cells intact in the eye by autoradiographic methods. The present proposed study represents an effort aiming toward understanding the dynamic relatioships that existing among the choroid the RPE and the retina, and the conditions that lead to breakdown of these relationships, such as the retinal detachment.