CONFORMATION OF MITOGENIC PEPTIDE GROWTH FACTORS

有丝分裂生长因子在关键的生物学作用中发挥着核心作用。 组织生长、发育、修复和维持的过程。 它们也与肿瘤的转化有关 疾病缠身的国家。这项研究的目标是确定 人表皮生长因子(HEGF)的构象研究 建立其结构和功能之间的关系。这个 在片段中保留关键结构特征的可能性 将被视为合理设计小型汽车的基础 分子激动剂或拮抗剂具有治疗作用 价值。在本研究中，高分辨率核磁共振的方法学 将使用光谱学，结合质子技术 同核二维核磁共振及其新技术 利用存在的其他原子核的信息。核磁共振 实验将与化学合成相结合，使用固体 多肽合成阶段，制备足够量的天然多肽 多肽、类似物和片段，并结合稳定 ~(13)C、~(15)N等同位素标记在核磁共振检测中的应用 构象。

Mitogenic growth factors play a central role in the key biological processes of tissue growth, development, repair and maintenance. They have also been associated with neoplastic transformation in diseased states. The goal of this study is determination of the conformation of human epidermal growth factor (hEGF) to try to establish a relationship between its structure and function. The possibility of preserving the key structural features in fragments would then be examined as a basis of rational design of small molecule agonists or antagonists that would have therapeutic value. In this study the methodology of high-resolution NMR spectroscopy will be used, combining the techniques of proton homonuclear 2-dimensional NMR with new techniques for exploiting information from other nuclei present. The NMR experiments will be combined with chemical synthesis, using solid phase peptide synthesis, to prepare sufficient amounts of native peptides, analogues, and fragments and to incorporate stable isotopic labels such as 13C and 15N as NMR probes in determining the conformation.