MARGINAL COPPER INTAKE EFFECTS--SOD & LIPID PEROXIDATION

许多美国人的膳食铜摄入量低于推荐量。 然而，这种边际铜摄入量的后果仍然是 下定决心。拟议的项目将检验边际的假设 大鼠体内的铜状态会产生足够低的肝脏活性 铜锌超氧化物歧化酶(SOD)浓度过高引起 是脂质过氧化的结果。铜锌超氧化物歧化酶清除超氧阴离子自由基 导致脂质过氧化，这一过程被认为是导致许多疾病的原因 以及包括癌症在内的伤害。将进行关于以下方面的研究： 单独的边缘铜状态，并结合3种应力状态中的1种 可降低大鼠肝脏铜锌超氧化物歧化酶活性(边际锌状态， 炎症和链脲佐菌素诱导的糖尿病)。老鼠也会被喂食 边际铜水平(2.5ppm铜)，或为比较目的，喂入铜 认为足够(百万分之六点零)或极差(百万分之零点二)的水平。在……里面 各饮食组大鼠分别加减应激状态、血脂 过氧化作用会被CC14激发。这种制剂经常被用来模拟 许多过氧化氢的产生状态都可能发生在人类身上。饮食将是 给雄性、断奶大鼠5或15周，或给成年大鼠5周 老鼠。每种方案的大鼠都或多或少地表现出对脂质的抵抗力。 比其他方案的大鼠更容易发生过氧化。由于脂质过氧化 可能发生在一个以上的亚细胞部位，并导致几种类型的 损害，这一过程将通过多项测量进行评估。 例子包括共轭二烯积累和苯肼反应。 不饱和脂肪酸水平。后者被认为与风险有关 癌症的发展。总而言之，这个项目将研究一个普通的人类 从肝脏铜锌超氧化物歧化酶来看，营养问题、边际铜状况 活性和脂质过氧化，2个生物学参数具有潜在的 广泛的健康影响。

Many Americans consume below recommended amounts of dietary copper. However, the consequences of such marginal copper intake remain to be determined. The proposed project would test the hypothesis that marginal copper status in rats can produce low enough hepatic activity concentrations of Cu-Zn superoxide dismutase (SOD) to cause high degrees of lipid peroxidation. Cu-Zn SOD eliminates superoxide radicals which can causes lipid peroxidation, process believed to contribute to many disease and injuries including cancer. Studies will be conducted on the effects of marginal copper status alone, and combined with 1 of 3 stress states which can reduce rat liver Cu-Zn SOD activities (marginal zinc status, inflammation and streptozotocin-induced diabetes). Rats will be fed either marginal copper levels (2.5 ppm Cu) or, for comparison purposes, fed copper level considered adequate (6.0 ppm) or extremely deficient (0.2 ppm). In some rats from each diet group, plus or minus the stress states, lipid peroxidation will be stimulated by CC14. This agent is often used to mimic many peroxide production states which could occur in humans. Diets will be given for 5 or 15 weeks to male, weanling rats, or for 5 weeks to adult rats. Rats in each protocol could show more or less resistance to lipid peroxidation than rats in the other protocols. Since lipid peroxidation can occur in more than one subcellular site and cause several types of damage, this process will be assessed by a number of measurements. Examples include conjugated diene accumulation and phenylhydrazine-reactive unsaturated fatty acids levels. The latter is considered relevant to risk of cancer development. In summary, this project would study a common human nutritional problem, marginal copper status, in terms of liver Cu-Zn SOD activities and lipid peroxidation, 2 biological parameters with potentially wide ranging health implications.