VIP RECEPTOR IN THE RETINAL PIGMENT EPITHELIUM

视网膜色素上皮(RPE)是单层的 脉络膜之间并列的特化神经上皮细胞 以及眼睛的视网膜。 几种荷尔蒙和神经递质存在于 脉络膜(11-14)或视网膜(15，16)可使 鸡视网膜色素上皮细胞内cAMP浓度的研究 胚胎。本研究建议审查这一可能性 脉络膜和/或脉络膜的激素和神经递质 视网膜起源可能是RPE功能的调节器。 在RPE的环状AMP池的调节器中， 血管活性肠肽(VIP)是其中最有效的一种。这个 因此，RPE上的VIP受体将被用作 确定荷尔蒙和 调节RPE功能的神经递质。 这项拟议的研究将描述血管活性肠肽受体在 以下方面与建立一个生理角色有关 RPE细胞中的这种受体： 1)VIP受体在根尖和/或根尖的定位 基侧膜， 2)受体的功能研究：血管活性肠肽对血管紧张素转换酶的影响 电解质和大分子的传输，3)机理 视网膜色素上皮细胞的血管活性肠化作用：超越腺苷的刺激 细胞蛋白的环化和磷酸化，4) 兔视网膜色素上皮细胞VIP受体的可视化研究 眼的放射自显影方法。 目前拟议的研究是一项旨在 理解存在的动态关系 脉络膜RPE和视网膜，以及导致 这些关系的破裂，例如视网膜脱离。

The retinal pigment epithelium (RPE) is a single layer of specialized neuro-epithelial cells juxtaposed between the choroid and the retina of the eye. Several hormones and neurotransmitters existing either in the choroid (11-14) or in the retina (15, 16) are found to elevate the intracellular cyclic AMP concentration of the RPE from the chick embryos. The present study propose to examine the possibility that the hormones and neurotransmitters of the choroidal and/or retinal origin may function as modulators of the RPE functions. Among the modulators of the cyclic AMP pools of the RPE, the vasocative intestinal peptide (VIP) is the most effective one. The VIP receptor on the RPE is therefore to be used as a model in identifying the roles played by the hormones and neurotransmitters in modulating the functions of the RPE. The proposed study will characterize the VIP receptor in the following aspects relevant to establishing a physiological role for this receptor in the RPE cells: 1) Localization of the VIP receptors on the apical and/or the basolateral membrane, 2) Functional studie of the receptors: the effect of VIP on transport of electrolyte and macromolecule, 3) The mechanism of VIP action of the RPE cells: beyond stimulation of the adenylate cyclase and phosphorylation of the cellular proteins, 4) Visualization of the VIP receptors on the RPE cells intact in the eye by autoradiographic methods. The present proposed study represents an effort aiming toward understanding the dynamic relatioships that existing among the choroid the RPE and the retina, and the conditions that lead to breakdown of these relationships, such as the retinal detachment.