CHEAS ROLE IN REGULATING CHEMOTACTIC SIGNAL TRANSDUCTION

这项建议侧重于Cheas在调节信号中所起的作用 细菌趋化作用中的转导。有证据表明，Cheas 与蛋白激酶Cheal和一种调节蛋白质相互作用 切尔活动，咀嚼。以确定CheAS是否调节 CHEAL，系留细胞分析将用于比较鞭毛 细胞的旋转行为，表达不同比例的Cheal和CheA。 为了确定CheA与之物理上相互作用的趋化蛋白， CheAS的显性突变等位基因将被分离和构象 确定了这些等位基因的抑制子。探索Cheas在人类免疫系统中的作用 调节磷酸盐转运，从零星提取物中提取蛋白质复合体 表达Cheal、CheA、Chew和转导蛋白亚群的细胞将是 用多克隆抗CHEA抗体进行免疫沉淀并测定 CHEAL从[伽马-32P]ATP到CHEAL的磷酸盐转移。除了……之外 有助于实现长期目标，即了解 信号转导通路相互关联，使细胞产生适当的 对环境刺激的反应，预计这项研究将有助于 了解不同形式的蛋白激酶所起的调节作用 参与信号转导，并对翻译和结构 同相重叠基因的蛋白质产物之间的关系。

This proposal focuses on the role of CheAS plays in regulating signal transduction in bacterial chemotaxis. Evidence suggests that CheAS interacts with the protein kinase, CheAL, and a protein that regulates CheAL activity, CheW. To determine whether CheAS regulates the activity of CheAL, tethered cell assays will be used to compare the flagellar rotational behavior of cells that express varying ratios of CheAL to CheAS. To identify chemotaxis proteins with which CheAS physically interacts, dominant mutant alleles of CheAS will be isolated and conformational suppressors of these alleles identified. To explore the role of CheAS in regulating phosphate transfer, protein complexes from extracts of null cells expressing subsets of CheAL, CheAS, CheW and transducers will be immunoprecipitated using polyclonal anti-CheA antibodies and assayed for phosphate transfer by CheAL from [gamma-32P] ATP to CheAL. In addition to contributing to the long-term goal of understanding how components of signal transduction pathways interrelate such that cells elicit proper responses to environmental stimuli, it is expected this study will help to understand the regulatory role played by alternate forms of protein kinases involved in signal transduction and to the translational and structural relationships between protein products of in-phase overlapping genes.