X-RAY STRUCTURAL STUDIES OF PROTEINS

1. DNA-蛋白质相互作用 第一个目的是更好地理解DNA-蛋白质的分子基础 识别和基因表达的调节。 噬菌体cro阻遏蛋白的研究将继续进行 λ，其三维结构已被确定。 在DNA结合和/或蛋白质中具有意外变化的突变cro蛋白质 还将从晶体学上研究稳定性。 将继续尝试确定cro络合物的结构 适当的DNA片段。 试图确定MerR金属调节的结构 蛋白质和其它DNA结合蛋白，以及这些蛋白质的复合物 蛋白质与DNA也将被追求。 2. 蛋白质折叠、功能和进化 第二个目标是更好地了解决定 蛋白质的结构、稳定性、动力学、折叠和活性。 正在进行的突变噬菌体的遗传、结构和热力学研究 溶菌酶，以及鹅溶菌酶，噬菌体P22的比较研究 溶菌酶和其他蛋白质，提供了一个非常有利的实验 解决这些问题的框架。

1. DNA-Protein Interaction The first aim is to better understand the molecular basis of DNA-protein recognition and the regulation of gene expression. Studies will be continued of the cro repressor protein from bacteriophage lambda, for which the three-dimensional structure has been determined. Mutant cro proteins with unexpected changes in DNA binding and/or protein stabilities will also be studied crystallographically. Attempts will be continued to determine the structure of cro complexed with appropriate DNA fragments. Attempts to determine the structures of the MerR metalloregulatory protein and other DNA binding proteins, as well as complexes of these proteins with DNA will also be pursued. 2. Protein Folding, Function, and Evolution The second goal is to better understand the factors that determine the structure, stability, dynamics, folding and activity of proteins. Ongoing genetic, structural and thermodynamic studies of mutant phage lysozymes, as well as comparative studies of goose lysozyme, phage P22 lysozyme, and other proteins, provide a very favorable experimental framework within which to address these problem areas.