DNA PROBES FOR THE DIAGNOSIS OF BACTERIAL VAGINITIS

五种细菌和一种支原体作为病原体的作用 细菌性阴道病（BV）的药物，是由越来越多的支持 科学数据。 标准方法不适用于常规 这种疾病的诊断。 16S的800 - 1，000个核苷酸 每种BV微生物的核糖体RNA（rRNA）将被 测序 将合成短DNA探针以检测 每种BV微生物的16S rRNA。 32P标记DNA探针 将与高分子量RNA和DNA杂交 分离自单个纯培养物。 杂交特异性 将在四乙基铵中评价每种DNA探针的 氯化物和四甲基氯化铵溶液， DNA中A-T相对于G-C碱基对的优先解链 杂交，产生只有链长的杂交 依赖。 在这些溶液中，可以使用相同的探针的混合物。 长度，但序列不同，将表现出相同的 杂交特性 这些盐溶液还将允许 同时使用多个探针（长度相同）， 允许开发通用杂交形式。 的 长期目标是提供快速准确的诊断 测试产品的BV。

The role of five bacteria and a mycoplasma as the etiologic agents of Bacterial Vaginosis (BV), is supported by growing scientific data. Standard methods are inadequate for the routine diagnosis of this disease. 800-1,000 nucleotides of the 16S ribosomal RNA (rRNA) of each BV microorganism will be sequenced. Short DNA probes will be synthesized to detect the 16S rRNA of each BV microorganism. 32P-labeled DNA probes will be hybridized against high molecular weight RNA and DNA isolated from individual pure cultures. Hybridization specificity of each DNA probe will be evaluated in tetraethylammonium chloride and tetramethylammonium chloride solutions to abolish the preferential melting of A-T versus G-C base pairs in DNA hybrids, yielding hybridizations which are only chain-length dependent. In these solutions, mixtures of probes of the same length, but differing in sequence, will exhibit identical hybridization properties. These salt solutions will also allow simultaneous use of multiple probes (of the same length) and will allow the development of a universal hybridization format. The long-term objective is to provide a rapid and accurate diagnostic test product for BV.