IMMUNOASSAYS FOR TAXOL

本项目的总体目标是生产选择性免疫测定 能够区别检测紫杉醇和紫杉醇的同类物， 用于检测常见紫杉烷环结构的通用免疫测定法。紫杉醇 目前正在进行二期临床试验。的HPLC方法 紫杉醇是可获得，但是用于相关化合物和代谢物的方法 没有被报道。拟议的免疫测定法将提供一种替代方法 紫杉醇及相关化合物的分析方法，可补充 临床研究中的HPLC方法学。此外，免疫测定 对紫杉醇特异性将有助于鉴定高产紫杉醇的 Taxis Brevifola的菌株。我们计划合成特征良好的紫杉醇 这些免疫原应该在兔子和小鼠中引发抗体应答。的 第一阶段研究的目标是证明以下方法的可行性： 利用多克隆兔产生紫杉醇特异性免疫测定 抗体的 第二阶段的研究将集中在完善紫杉醇特异性测定和 产生用于相关的额外的基于单克隆抗体的免疫测定 紫杉烷类

The overall goal of this project is to produce selective immunoassays capable of differentially detecting taxol and congeners of taxol, and a generic immunoassay for detecting the common taxane ring structure. Taxol is currently showing promise in Phase II clinical trials. HPLC methods for taxol are available but methods for related compounds and metabolites have not been reported. The proposed immunoassays would provide an alternative analytical method for taxol and related compounds, which could complement HPLC methodology in clinical studies. In addition, the immunoassays specific for taxol would be useful for identifying high taxol producing strains of Taxis Brevifola. We plan to synthesize well characterized taxol immunogens which should elicit antibody responses in rabbits and mice. The goal of Phase I research will be to demonstrate the feasibility of producing a taxol-specific immunoassay utilizing polyclonal rabbit antibodies. Phase II research will focus on refining the taxol-specific assay and on production of additional monoclonal antibody-based immunoassays for related taxanes.