ELECTRICAL MEASUREMENT OF CELL MOTILITY IN VITRO

我们一直在开发一种仪器， 在组织培养中，并打算制造一种适用于 医学/生物学研究市场。 当前的挑战是 通过这种方法测量的细胞运动到其他识别的细胞 功能协调发展的 在这项研究中，癌细胞系的运动测量 电将与通过常规测量的运动性相关。 方法和已知的细胞转移能力。 在这种新的仪器方法中，细胞在小金 带有微弱交流电流的电极。 细胞的运动是 反映在测量的电极阻抗波动中，并且可以 在稀疏和汇合的细胞层中均检测到。 实验表明 远超光学显微镜分辨率的运动检测。 具体而言，将在六个 Dunning大鼠前列腺腺癌细胞的亚系。 这些数据将 通过各种算法进行统计处理，并与视频进行比较 来自约翰霍普金斯肿瘤中心合作者的显微镜数据。 他们 已经发现细胞运动的某些方面与 这些细胞的转移。

We have been developing an instrument that electrically detects cell motion in tissue culture and intend to make a commercial device suitable for the medical/biological research market. The immediate challenge is to relate motion of cells measured by this method to other recognized cellular functions. In this study, motion of cancer cell lines measured electrically will be correlated with motility measured by conventional means and with the known metastatic abilities of the cells. In this new instrumental method, cells are cultured on small gold electrodes carrying weak AC currents. The motions of the cells are mirrored in the measured electrode impedance fluctuations and can be detected in both sparse and confluent cell layers. Experiments show detection of movements well beyond the resolution of an optical microscope. Specifically, extensive impedance measurements will be carried out on six sublines of Dunning rat prostatic adenocarcinoma cells. These data will be statistically processed by a variety of algorithms and compared with video microscopy data from collaborators at Johns Hopkins Oncology Center. They have found strong correlations of some aspects of cell motility with metastasis for these cells.