PRODUCTION OF SPONGE NATURAL PRODUCTS VIA CELL CULTURE

本项目的长期目标是发展海绵细胞培养 满足和刺激对海绵化合物需求的方法 用于药物发现和开发： 1.代谢物表达系统提供小样本（<1 mg）， 发现研究 2.优化方案的发展，高产量，化合物- 支持发展研究的具体生产系统。 3.供应化学复杂分子的受控制造系统 用于后期开发和营销。 这些技术将共同实现一系列增值步骤， 每种海绵衍生药物的成本效益和及时的基础。 目前， 海绵天然产品的开发依赖于海洋采集 往往是困难的或不可能的。 发展治疗学 NCI计划测试数千种海绵衍生代谢物 在接下来的几年里，已经报道了海绵代谢物， 抗艾滋病活动。 制药企业也在积极评估 海绵代谢物。 该项目将开发支持这一点的技术 工作，并确保根据需要提供足够的临床用品。 拟议的第一阶段研究将评估申请的可行性， 微小的修改，现有的培养和冷冻保存技术 哺乳动物和海绵细胞。

The long-term objective of this project is to develop sponge cell culture methods that will satisfy and stimulate demand for marine sponge compounds for drug discovery and development: 1. Metabolite expression systems to provide small samples (<1 mg) for discovery research. 2. Optimization protocols for the development of high-yield, compound- specific production systems to support development research. 3. Controlled manufacturing systems to supply chemically-complex molecules for late development and marketing. Together these technologies will enable a series of value-added steps for each sponge-derived drug on a cost-effective and timely basis. At present, development of sponge natural products is dependent on ocean collections that are often difficult or impossible. The Developmental Therapeutics Program of the NCI plans to test thousands of sponge-derived metabolites over the next few years and has already reported sponge metabolites with antiAIDS activities. Pharmaceutical companies are also actively evaluating sponge metabolites. This project will develop technologies to support this work and ensure that adequate clinical supplies are available as needed. The proposed Phase I study will evaluate the feasibility of applying, with minor modifications, extant technologies for culture and cryopreservation of mammalian and sponge cells.