CONSTRUCTION AND CLINICAL TRIALS OF 31P NMR SPECTROMETER

3.0特斯拉高分辨超导的设计与建造 带有短水平30厘米内径的磁体系统，可与 磷能量公司磷核磁共振 分光计。 宾夕法尼亚大学已经进行了初步工作， 使用1.5和1.9特斯拉超导磁体的生物化学系 用于各种动物模型和疾病的体内研究 人类。与这项建议相关的是对动物模型肿瘤和 其治疗方法是非侵入性地跟随他们的时间进程。此外, 肝脏神经母细胞瘤曾在婴儿和儿童中进行过研究 以及成年人四肢上的肿瘤。 与1H核磁共振成像不同的是，1H核磁共振成像产生的解剖数据有限 特异性，31P核磁共振直接测量新陈代谢之前 解剖上的变化。不幸的是，31便士的价格非常低。 与100米处的质子相比，浓度为10-20毫米，因此 更难察觉。 P-E公司率先将31P核磁共振仪器应用于活体 各种代谢紊乱的测量。最近的高分辨率 质子核磁共振，其中主体水信号被抑制，产生潜在的 相同磷代谢产物加乳酸盐的高分辨光谱 已经表明，对31P和高分辨率1H的观测将产生 更多关于肿瘤新陈代谢的有用信息。

Design and construction of a 3.0 Tesla high resolution superconducting magnet system with short horizontal 30cm bore to incorporate with Phospho-Energetics, Inc. phosphorous nuclear magnetic resonance spectrometer. Preliminary work has been performed at the University of Pennsylvania, Department of Biochemistry using 1.5 and 1.9 Tesla superconducting magnets for variety of in vivo studies of various diseases in animal model and humans. Relevant to this proposal are studies in animal model tumors and therapy thereof to follow their time courses non invasively. In addition, neuroblastoma tumors of the liver have been studied in infants and children as well as tumors on limbs of adults. Unlike 1H NMR imaging which yields anatomical data with limited specificity, 31P NMR yields direct measurement of metabolism that precedes anatomical changes. Unfortunately, 31P is present in very low concentrations, 10-20mM compared to protons at 100M, and is therefore much more difficult to detect. P-E has pioneered in the application of 31P NMR instrumentation to in vivo measurements of various metabolic disorders. More recently high resolution proton NMR where the bulk water signal is suppressed yielding underlying high resolution spectra of the same phosphorous metabolites plus lactate has indicated that observation of 31P and high resolution 1H would yield more useful information on the metabolism of tumors.