LIPOPHILIC LI+ ION FLUOROPHORES

我们建议合成一类新的亲脂性锂离子 荧光团最终可能会在各种 细胞生理学家和神经科学家感兴趣的应用。 它们将能够在细胞膜上以锂离子的形式发挥作用 载体将Li+转运到细胞质中用于研究 抑制肌醇磷酸代谢。做了一些改动 在取代基团中，它们可能有助于确定 胞质锂离子浓度和/或直接 锂离子的时空变化观测 单个细胞中的浓度。它们也可以在体外用来 接受治疗的患者血清中锂的测定 躁狂/抑郁性精神病。固定化荧光团的研究 光纤传感器的终结最终可能导致体内 血液中锂离子的测定。在第一阶段，我们打算合成 这组荧光团中最简单的成员是测试 我们提出的合成路线，并确定它是否会 在体外可作为Li+离子的选择性荧光团。 在第二阶段，我们将用荧光团合成其他类似物。 具有长波长发射和更强的疏水性。我们 还将探索它们在体外检测中的可能用途 血清中Li+的测定，以及胞内游离Li+的测定 活细胞中的离子浓度。

We propose to synthesize a new family of lipophilic Li+ ion fluorophores which may ultimately find use in a variety of applications of interest to cell physiologists and neuroscientists. They will be able to function in cell membranes as lithium ion carriers to transport Li+ into the cytoplasm for study of the inhibition of inositol phosphate metabolism. With some alterations of substituent groups, they may be useful to determine the concentration of cytosolic lithium ion and/or for direct observation of temporal and spacial changes in Li+ ion concentrations in single cells. They may also be used in vitro to determine lithium in the blood serum of patients being treated for manic/depressive psychosis. Immobilization of the fluorophore on the end of a fiber optic sensor could eventually lead to in vivo determination of Li+ in blood. In Phase I, we intend to synthesize the simplest member of this group of fluorophores to test the synthesis route we have proposed, and to determine whether it will function in vitro as a selective fluorophore for Li+ ion. In Phase II we will synthesize other analogs with fluorophores having long wavelength emissions and greater hydrophobicity. We will also explore their possible use for the in vitro determination of Li+ in blood serum, and for determination of cytosolic free Li+ ion concentration in viable cells.