ANALYSIS OF DIGOXIN IN USING A SIMPLE IMMUNOASSAY

这项工作的长期目标是开发一种快速，比色 用于洋地黄成分的仪器独立测定，特别是 地高辛和洋地黄毒苷。 Genesis Labs的“IMMUNOSTRIP”试验（受 专利）被构造成一种配置，该配置适用于 快速检测生物流体例如尿中的药物和代谢物， 血液或唾液 这种方法相对简单，说明它很容易 由医学上不成熟的人使用。 我们先尝试一下， 测定血清中地高辛浓度为5 ng/ml。 最终，我们的目标 将测定系统的灵敏度提高到1-5 ng/ml 范围 这将需要使用手持式反射计（每台1000美元 当前的单元）用于阅读条带，然而，通过改变 抗原和酶修饰的抗地高辛抗体的量 IMMUNOSTRIP，我们可以在此较低范围内进行目视检测。 该测定具有许多用于监测治疗药物的应用，包括 地高辛，因此将对我们的知识产生重大影响， 健康利益/风险比在我们的社会人口谁需要 毒品 基于我们监测其他激素、药物和小 半抗原，我们相信，在未来，通过使用 高度特异性的多克隆抗血清或更可能的单克隆抗体， 可以监测活性地高辛， 在医生办公室环境中地高辛的治疗水平与毒性水平 一分钟的测试。

The long term objectives of this work is to develop a rapid, colorimetric instrument independent assay for cardio digitalis constituents, notably digoxin and digitoxin. Genesis Labs' "IMMUNOSTRIP" assay (protected by patent) is constructed in a configuration, which is applicable for the rapid detection of drugs and metabolites in biological fluids e.g. urine, blood or saliva. The relative simplicity of the method suggests its easy use by medically unsophisticated individuals. We will attempt initially, to determine digoxin in serum at a level of 5 ng/ml. Ultimately, our aim is to increase the sensitivity of the assay system into the 1-5 ng/ml range. This will require the use of a hand held reflectometer ($1000 per unit currently) for reading the strips, however it is feasible by varying the amount of antigen and enzyme modified anti-digoxin antibody within the IMMUNOSTRIP that we can produce a visual assay within this lower range. The assay has many applications for monitoring therapeutic drug including digoxin, and therefore will have a major impact on our knowledge of the health benefit/risk ratio within populations of our society who require drugs. Based on our ability to monitor other hormones, drugs and small haptens using this assay, we feel confident that in the future by using highly specific polyclonal antisera or more likely monoclonal antibodies, it may be possible to monitor active digoxin and thereby predict therapeutic versus toxic levels of digoxin in a physician's office setting with a one minute test.