OPTICAL ASSAY FOR OXYGEN IN DIAGNOSTIC TEST

该项目的长期目标是开发一种仪器和分析方法 氧的体外光学测量系统，将用于 涉及消耗氧气的反应物的诊断测试。最直接的 建议的应用是使用氧化酶进行胆固醇分析 系统。其概念是氧气的利用直接与 因此，测定底物周转量的变化量 样品液中的氧气可以准确地测量底物 集中精神。 初步结果表明，这种光学传感方法具有很好的应用前景。 并可以作为系统的基础，方便地处理多个 样本。第一阶段的目标是彻底评估范围、准确性 和可靠性，以确定其可行性 胆固醇分析。在这一测试阶段，已知的胆固醇样本 值将与常规酶试剂反应，其变化 样品的氧含量将用光纤氧气进行跟踪 传感器。这些数据将与已知的胆固醇进行比较 水平以及其他常规检测方法的结果。 该系统的技术旨在解决准确性和可靠性问题 小型办公室、诊所和筛查现场测试环境中的问题， 提供了比现有检测方法更多的优势。替代站点测试市场是 对高质量的医疗保健很有价值。胆固醇问题尤其令人担忧 对于冠心病来说很重要，并构成了一个很大的 诊断市场。通过在现场提供经济实惠的质量测试， 该系统将对医疗服务的提供产生广泛的好处。

The project's long term objective is to develop an instrument and assay system for optical measurement of oxygen in vitro that will be used for diagnostic tests involving reactants that consume oxygen. The immediate application proposed is for cholesterol analysis using an oxidative enzyme system. The concept is that oxygen utilization is directly tied to substrate turnover, therefore, determination of the change in the amount of oxygen in a sample fluid can be an accurate measure of the substrate concentration. Preliminary results indicate that the optical sensing approach has promise and could be the basis for a system that conveniently processes multiple samples. The aim of Phase I is to thoroughly assess the range, accuracy and reliability, of the approach in order to establish its feasibility for cholesterol analysis. In this test phase, cholesterol samples of know values will be reacted with conventional enzyme reagent, and the change in oxygen content of the sample will be tracked with fiber optic oxygen sensors. The data will be compared and contrasted to the know cholesterol levels as well as the results of other conventional assay methods. The system's technology is designed to address accuracy and reliability issues in the small offices, clinics, and screening-site test settings, offering advantages over existing assay. The alternate site test market is valuable for quality health care. Cholesterol concerns are particularly important regarding coronary heart disease, and constitute a large diagnostic market. By providing economical quality testing on site, this system will produce widespread benefits to health care delivery.