DEVELOPMENT OF A HYBRID ARTIFICIAL ORGAN

这项研究的目标是确定使用稠密、 免疫隔离异物的半透性聚合物膜 混合型人造器官。将合成一系列聚合物，并 具有渗透性、细胞毒性和生物稳定性。聚合物 组成/形态将发生变化，以产生一系列具有 逐步增加渗透率。膜将从溶液中浇铸出来 在均匀条件下使用实验室卷筒纸涂布机涂布到离型衬垫上 空气流动和热历史。相同的铸造液将被用于 制备小直径中空纤维膜用作细胞包装 小鼠植入和体外细胞生长实验。使用一系列 永久性溶液，平板膜对水的渗透性， 葡萄糖和几种蛋白质将在非稳定状态下进行测量 扩散实验。将使用灵敏的量热/染色方法 为了定量葡萄糖和蛋白质穿透细胞膜 选定永久/膜的渗透系数的计算 成对的。在第二阶段，我们将建造和植入混合器官， 保护性、选择性可渗透的屏障，用于培养和/或遗传 基因工程细胞。在第三阶段，第二阶段开发的膜将是 由我们的许可证持有人作为混合人造产品的(组件)商业提供 器官。

The goal of this study is to determine the feasibility of using a dense, semipermeable polymer membrane for immuno-isolation of foreign tissue in a hybrid artificial organ. A series of polymers will be synthesized and characterized for permeability, cytotoxicity and biostability. The polymer composition/morphology will be varied to yield a series of polymers with stepwise increasing permeability. Membranes will be cast from solution onto release liner, using a laboratory web coater under uniform conditions of air flow and thermal history. The same casting solution will be used to prepare small-diameter, hollow-fiber membranes for use as cell packets for mouse implant and in vitro cell growth experiments. Using a series of permanent solutions, the permeability of the flat membranes to water, glucose and several proteins will be measured in a non-steady-state diffusion experiment. Sensitive calorimetric/staining methods will be used to quantify glucose and proteins which permeate the membranes for calculation of permeability coefficients for selected permanent/membrane pairs. In Phase II we will build and implant hybrid organs with protective, selectively-permeable barriers for cultured and/or genetically engineered cells. In Phase III the membranes developed in Phase II will be offered commercially by our licensee as (components of) hybrid artificial organs.