MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM

我们建议购买蔡司EM 902传输电子设备 带有投射能量滤光片的显微镜，以取代磨损的显微镜 JEOL JEM100B-3，一直在服务于 生物物理研究部的教职员工和 密歇根大学湖泊研究部。这个 现有的显微镜在1985年的一半时间里一直无法操作， 导致至少一个研究项目中断和 其他项目的异地执行情况。这台新仪器将 用于可溶蛋白质晶体的常规显微镜观察， DNA和分离得到的海胆组蛋白基因染色质。低剂量 葡萄糖包埋和冷冻水化周期的显微镜观察 膜结合蛋白的阵列也在计划中。进阶 负染标本和冷冻水化标本的显微镜观察 染色质将被用来直接检测和提炼一种最近 提出了染色体纤维内部结构的模型。 通过消除非弹性，将获得更大的对比度 电子，它主宰着冷冻分子的图像。 电子光谱成像将被用来确定 染色质纤维中蛋白质和DNA的径向分布。在……里面 此外，非弹性暗场显微镜将被开发用于 未染色生物分子的定量质量分析--a 由两名教职员工支持的独特应用程序 卓有成效地使用扫描透射电子显微镜 布鲁克海文国家实验室的设施，以确定 染色体纤维和酶中的质量分布。 该显微镜将放置在生物物理研究中心 密歇根大学的部门，这将确保其 独特的能力将被充分利用，用于生化， 分子水平上的生物物理学和细胞生物学研究。

We propose to purchase a Zeiss EM 902 transmission electron microscope with Castaing energy filter, to replace a worn-out JEOL JEM100B-3 that has been serving the day-to-day needs of the faculty of the Biophysics Research Division and the Great Lakes Research Division of The University of Michigan. The existing microscope has been inoperable during half of 1985, causing discontinuation of at least one research project and the performance of other projects off-site. The new instrument will be used for routine microscopy of crystals of soluble proteins, DNA, and isolated sea urchine histone gene chromatin. Low dose microscopy of glucose-embedded and frozen-hydrated periodic arrays of membrane-bound proteins are also planned. Advanced microscopy of negatively-stained and of frozen-hydrated chromatin will be used to directly test and refine a recently proposed model for the internal structure of chromosome fibers. Greater contrast will be achieved by elimination of the inelastic electrons, which dominate the images of frozen molecules. Electron spectroscopic imaging will be used to determine the radial distribution of protein and DNA in chromatin fibers. In addition, inelastic dark field microscopy will be developed for quantitative mass analysis of unstained biological molecules -- a unique application supported by two faculty who have productively used the scanning transmission electron microscopy facilities at Brookhaven National Laboratory to determine the distribution of mass within chromosome fibers and enzymes. The microscope will be located in the Biophysics Research Division of The University of Michigan, which will assure that its unique capabilities will be fully exploited for biochemical, biophysical, and cell biological studies at the molecular level.