EKS CENTER REQUEST FOR VG MM707E MS WITH LC INTERFACE

本提案中要求的仪器将提供一个独特的 一组NIH支持的研究人员的能力，他们有一个共同的 需要高灵敏度的分析和结构表征 复合脂质和寡糖的方法。 PI和合作者， 使用现有的Finnigan 4500质谱仪和移动带液体 色谱接口，已经证明，在线LC/MS 分析未衍生化的磷脂和糖脂可以提供快速的 分析和结构信息以前不可能。 质量 Finnigan 4500（1000 u）的射程是对 开发LC/MS用于分子量大于1000的复合脂质 一千道尔顿。 此外，还迫切需要获得现有的 正在进行的研究计划的质谱仪设施。 拟议 采购VG MM 707 E（以前称为7070 E-HF）大质量 具有DCI和LC接口的范围质谱仪（HF-MS）将提供 在波士顿地区的高质量在线LC/MS的独特能力 分析和带式FAB电离或源内闪蒸。 的 现有的仪器将被释放，用于需要MS的研究计划 低分子量材料的分析。 新仪器将 直接和独特地支持研究计划的需求， 应用，即方法开发（McCluer等人）、分析和 中性鞘糖脂的结构解析（Schwarting，McCluer 和Raghavan），神经节苷脂（McCluer，Jungalwala和Schwarting）复合物 异染性白质营养不良（Kolodny）中的磺基糖脂，磷脂 与脑髓鞘生成和发育的调节相关的分析 肿瘤（Jungalwala）和甘露糖苷相关寡糖分析 （丹尼尔）。 因此，该共享先进的HF-MS与DCI和LC接口 能力将服务于需求，并促进这些正在进行的 美国国立卫生研究院支持的研究项目，无疑将有助于 追求前所未有的新研究目标。

The instrumentation requested in this proposal will provide a unique capability for a group of NIH supported investigators who have a common need for highly sensitive analytical and structural characterization methods for complex lipids and oligosaccharides. The PI and collaborators, with the existing Finnigan 4500 mass spectrometer and a moving belt liquid chromatographic interface, have demonstrated that the on-line LC/MS analysis of underivatized phospholipids and glycolipids can provide rapid analytical and structural information previously not possible. The mass range of the Finnigan 4500 (1000 u) is a severe limitation to the exploitation of LC/MS for complex lipids which have molecular weights above 1000 daltons. There are also pressing demands for access to the existing mass spectrometry facilities from ongoing research programs. The proposed acquisition of a VG MM707E (formerly referred to as 7070E-HF) high mass range mass spectrometer (HF-MS) with DCI and LC interface will provide a unique capability in the Boston area for on line LC/MS with high mass analysis and belt FAB-ionization or in-source flash evaporation. The existing instrumentation will be freed for research programs requiring MS analysis of low molecular weight materials. The new instrumentation will directly and uniquely support the needs of research programs described in the application i.e. methods development (McCluer, et al), analysis and structural elucidation of neutral glycosphingolipids (Schwarting, McCluer and Raghavan), gangliosides (McCluer, Jungalwala and Schwarting) complex sulfoglycolipids in metachromatic leucodystrophy (Kolodny), phospholipid analysis related to regulation of myelinogenesis and development of brain tumors (Jungalwala) and oligosaccharide analysis related to mannosidoses (Daniel). Thus, this shared advanced HF-MS with DCI and LC interfacing capability will serve the needs and enhance the progress of these ongoing NIH supported research programs and will undoubtedly contribute to the pursuit of new research goals previously unapproachable.