STAGE SPECIFIC EMBRYONIC ANTIGEN-1 GLYCOCONJUGATES IN DEVELOPING RAT CEREBELLUM

大鼠小脑发育阶段特异性胚胎抗原-1 糖复合物

基本信息

项目摘要

Evidence that fucosylated glycoconjugates play a significant role in brain development is suggested by reports of severe mental retardation in patients that lack fucose residues. However, the mechanisms by which fucosylated glycoconjugates influence brain development are not known. The epitope 3-fucosyl-N-acetylactosamine (SSEA-1) is an excellent candidate to play a vital role in brain development, both because it is expressed in developing brain with temporal and spatial specificity, and because it has been shown to have a key role in cell-cell interactions in other tissues. Thus, we postulate that SSEA-1 plays a key role in the cell-cell interactions required for normal neural development. Our preliminary results indicate that SSEA-1 glycolipid expression in neonatal cerebellum correlates with granule cell migration, and the goal of this project is to elucidate the role of these molecules in this process. Specifically, we will characterize and measure SSEA-1 glycosphingolipids and proteins in developing rat cerebellum and relate these data to the stage and regional immunohistochemical appearance of this antigen. We also will characterize the fucosyltransferase activities involved in SSEA-1 glycoconjugate synthesis during cerebellar development, as our data suggests fucosyltransferase(s) play a critical role in controlling SSEA-1 glycolipid expression. Simultaneously, we will characterize the genes responsible for these fucosyltransferase activities and evaluate their regulation during development. Results obtained from these studies will then enable us to investigate the biological role of SSEA-1 glycoconjugates. We propose to determine whether overexpression, or blocking the accessibility, of SSEA-1 antigens in the neonatal cerebellum would result in anomalous behavior of developing granule cells. To cause over expression of SSEA-1, we propose to inject intracerebrally neonatal rats with AAV vectors carrying an epitope-tagged form of the relevant cloned rat fucosyltransferase under the control of a strong non-tissue specific promoter. To block the function of SSEA-1, we plan to inject irradiated hybridoma cells producing anti-SSEA-1 antibodies. These brains will then be examined histologically during subsequent development to establish the degree of fucosyltransferase and SSEA-1 expression and to determine whether perturbing SSEA-1 expression or availability changes the normal pattern of granule cell migration, parallel fiber extension and/or synaptogenesis.
证据表明,集中的糖缀合物在

项目成果

期刊论文数量(0)
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ROBERT H MC CLUER其他文献

ROBERT H MC CLUER的其他文献

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{{ truncateString('ROBERT H MC CLUER', 18)}}的其他基金

STAGE SPECIFIC EMBRYONIC ANTIGEN-1 GLYCOCONJUGATES IN DEVELOPING RAT CEREBELLUM
大鼠小脑发育阶段特异性胚胎抗原-1 糖复合物
  • 批准号:
    6301842
  • 财政年份:
    1999
  • 资助金额:
    $ 11.45万
  • 项目类别:
STAGE SPECIFIC EMBRYONIC ANTIGEN-1 GLYCOCONJUGATES IN DEVELOPING RAT CEREBELLUM
大鼠小脑发育阶段特异性胚胎抗原-1 糖复合物
  • 批准号:
    6108184
  • 财政年份:
    1998
  • 资助金额:
    $ 11.45万
  • 项目类别:
STAGE SPECIFIC EMBRYONIC ANTIGEN-1 GLYCOCONJUGATES IN DEVELOPING RAT CEREBELLUM
大鼠小脑发育阶段特异性胚胎抗原-1 糖复合物
  • 批准号:
    6240771
  • 财政年份:
    1996
  • 资助金额:
    $ 11.45万
  • 项目类别:
SMALL INSTRUMENTATION GRANT
小型仪器补助金
  • 批准号:
    3526215
  • 财政年份:
    1991
  • 资助金额:
    $ 11.45万
  • 项目类别:
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
少数民族高中生研究学徒计划
  • 批准号:
    2282554
  • 财政年份:
    1986
  • 资助金额:
    $ 11.45万
  • 项目类别:
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
少数民族高中生研究学徒计划
  • 批准号:
    3512564
  • 财政年份:
    1986
  • 资助金额:
    $ 11.45万
  • 项目类别:
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
少数民族高中生研究学徒计划
  • 批准号:
    3512563
  • 财政年份:
    1986
  • 资助金额:
    $ 11.45万
  • 项目类别:
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
少数民族高中生研究学徒计划
  • 批准号:
    3512562
  • 财政年份:
    1986
  • 资助金额:
    $ 11.45万
  • 项目类别:
EKS CENTER REQUEST FOR VG MM707E MS WITH LC INTERFACE
EKS 中心要求配备 LC 接口的 VG MM707E MS
  • 批准号:
    3519233
  • 财政年份:
    1985
  • 资助金额:
    $ 11.45万
  • 项目类别:
MICRO-HPLC & LC/MS METHODS FOR NEURAL SYSTEMS
微量高效液相色谱法
  • 批准号:
    3396894
  • 财政年份:
    1980
  • 资助金额:
    $ 11.45万
  • 项目类别:

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