ALDOSE REDUCTASE AND DIABETIC COMPLICATIONS

醛糖还原酶是多基因醛酮还原酶家族的成员， 与糖尿病并发症的病因学有关，这种并发症可以 影响许多组织，尤其是眼睛、神经和肾脏。最新版本 醛糖还原酶的研究，包括醛糖还原酶的开发 抑制剂作为潜在的治疗药物，已利用动物来源的 醛糖还原酶，其性质与人的醛糖显著不同 还原酶。最近，人们开发了一种方法，可以将人类 不改变其天然性质的醛糖还原酶。这个 本研究的目的是分析药物的动力学和结合作用。 人醛糖还原酶的性质为了建立一家公司 开发未来治疗药物的酶学基础。 具体目标如下： 1.测定多种形式的人醛糖还原酶 代表不同的基因产物。 2.分析多种形式的人体醛糖的动力学特性 以葡萄糖为底物的还原酶。 3.分析人醛糖还原酶的药物结合特性。

Aldose reductase, a member of the multi-gene aldo-keto reductase family, has been implicated in the etiology of diabetic complications which can affect many tissues, but especially eye, nerve and kidney. Most previous studies of aldose reductase, including development of aldose reductase inhibitors as potential therapeutic drugs, have utilized animal sources of aldose reductase, whose properties differ markedly from human aldose reductase. Recently, methods were developed that allow isolation of human aldose reductase without alteration of its native properties. The objective of the study is to analyze the kinetic and drug binding properties of human aldose reductase in order to establish a firm enzymological foundation upon which to develop future therapeutic drugs. The Specific Aims are as follows: 1. To determine whether the multiple form of human aldose reductase represent different gene products. 2. To analyze the kinetic properties of the multiple forms of human aldose reductase with emphasis on glucose as substrate. 3. To analyze the drug binding properties of human aldose reductase.