ETHANOL AND CELLULAR CALCIUM METABOLISM

最近的研究表明，几种细胞类型， 细胞内钙对激素或神经递质的反应 还水解磷脂酰肌醇4，5-二磷酸， 甘油二酯和三磷酸肌醇（IP 3）。 这可能介导 细胞内钙的动员。 在本研究中， 我们研究了IP 3参与的钙动员， 大鼠脑微粒体。 乙醇诱导的神经递质释放 未受刺激的突触体被发现是独立的 突触体外钙浓度 因此，影响 酒精对微粒体钙摄取和释放的影响 研究了 IP 3引起大脑中钙的快速释放 微粒体 体外添加100 mM乙醇对 ATP依赖的钙在微粒体中的积累，但相同的 乙醇释放的浓度占总累积量的25 微粒体中的钙 乙醇诱导的钙释放 在30 mM至500 mM范围内以浓度依赖性方式 毫米 随着醇含量的增加，钙的释放量增加。 乙醇的影响是温度依赖性的，这表明 钙转运的扩散控制过程。 这些结果 表明乙醇对静息释放的刺激作用 大鼠脑内神经递质的变化可能是由于微粒体 释放钙。 这个项目已经终止。

Recent studies show that several cell types that mobilize intracellular calcium in response to hormones or neurotransmitters also hydrolyze phosphatidylinositol 4,5-bisphosphate to produce diacylglycerol and inositol trisphosphate (IP3). which may mediate the mobilization of intracellular calcium. In the present study, we investigated the involvement of IP3 in calcium mobilization in rat brain microsomes. Ethanol-induced neurotransmitter release in unstimulated synaptosomes was found to be independent of the extrasynaptosomal calcium concentration. Therefore, the effects of alcohol on microsomal calcium uptake and release were also studied. IP3 caused a rapid release of calcium from brain microsomes. In vitro addition of 100 mM ethanol had no effect on ATP-dependent calcium accumulation in the microsomes, but the same concentration of ethanol released 25% of the total accumulated calcium from the microsomes. Ethanol induced calcium release in a concentration-dependent manner over the range of 30 mM to 500 mM. The amount of calcium release increased with higher alcohols. The effect of ethanol was temperature-dependent, suggesting a diffusion-controlled process for calcium transport. These results indicate that the stimulatory effect of ethanol on resting release of neurotransmitters in rat brain may be due to the microsomal release of calcium. This project has been terminated.