DESATURATION OF ESSENTIAL FATTY ACIDS USING STABLE ISOTOPE GC-MS

脱饱和度和伸长率是改性过程中的必要步骤 必需脂肪酸)、亚油酸(18：2n6)和亚麻酸(18：3n3) 酸，至花生四烯酸(20：4n6)和二十二碳六烯酸(22：6n3)， 分别进行了分析。后两种脂肪酸的水平降低了 动物的血细胞、肝脏和脑中的乙醇消耗。我们有 显示了破坏必需脂肪的潜在机制 这些组织中的酸度部分是由于抑制了 酒精对18碳必需脂肪酸的伸长/减饱和作用。一个 稳定同位素气相色谱-质谱仪(GC-MS)方法 已经开发出允许在体内检查这些过程的技术。 这种方法提高了大型动物研究的敏感性。 对小鼠、大鼠、猫和恒河猴的研究表明，所有 哺乳动物具有伸长和降低饱和度的能力，而后代的产品 可以在血液供应中进行监测。猫科动物，已经被给予了 酒精14周，在20：4n6中显示减少了80% 肝脏和血液中的二十碳五烯酸(20：5n3)以及 18：2N6和18：3N3的代谢率降低 20：4n6和20：5n3。我们的发现表明猫科动物的大脑 是这个物种产生22：6n3的一个独特的位置，因此，我们是 慢性酒精暴露对脑组织脂质影响的研究 组成和22：6N3组。我们最近的研究表明， 饮食中的脂肪调节人体内多不饱和脂肪酸的合成 食肉动物和非人类灵长类动物。我们已经启动了人体试验来 研究饮食和戒酒对必需脂肪的影响 酸代谢。

Desaturation and elongation are necessary steps in the modification of the essential fatty acids EFA), linoleic (18:2n6) and linolenic (18:3n3) acids, to arachidonic (20:4n6) and docosahexaenoic (22:6n3) acids, respectively. The levels of the latter two fatty acids are lowered by ethanol consumption in blood cells, liver and brain in animals. We have shown that the mechanisms underlying the disruption of essential fatty acid levels in these tissues are in part due to the inhibition of the elongation/desaturation of 18-carbon essential fatty acids by alcohol. A stable isotope gas chromatography-mass spectrometry (GC-MS) method has been developed that allows for the in vivo examination of these processes. This method has improved the sensitivity of studies in large animals. Studies in mice, rats, cats and rhesus monkeys have suggested that all mammals have elongation and desaturation capability and that heir products may be monitored in the blood supply. Felines, which had been given alcohol for 14 weeks, showed an 80% reduction in 20:4n6 and eicosapentaenoic (20:5n3) acid in the liver and blood together with a decrease in the metabolism of deuterated 18:2n6 and 18:3n3 to deuterated 20:4n6 and 20:5n3, respectively. Our findings show that the feline brain is a unique site of 22:6n3 production in this species, therefore, we are investigating the effects of chronic alcohol exposure on the brain's lipid composition and 22:6n3 formation. Our recent studies indicate specific fats in the diet regulate the synthesis of polyunsaturated fatty acids in carnivores and non-human primates. We have initiated human trials to investigate the effects of diet, and alcohol withdrawal on essential fatty acid metabolism.