DESATURATION OF ESSENTIAL FATTY ACIDS USING STABLE ISOTOPE/MASS SPECTROMETRY

去饱和度是必需脂肪修饰的必要步骤 将亚油酸和亚麻酸转化为花生四烯酸和二十二碳六烯酸， 分别进行了分析。酒精可以降低这些脂肪酸的水平。 对血细胞、肝脏和大脑的消耗。其背后的机制是 这些组织中必需脂肪酸水平的破坏在很大程度上是 未知。酒精引起的这种缺陷的一个可能的解释是 对18碳必需脂肪酸伸长/脱饱和的抑制作用 酒精。稳定同位素气相色谱/质谱仪(GC/MS) 已经开发出一种方法，允许在体内检查这些 流程。这项技术通过以下方式提高了方法的灵敏度 几个数量级，并使非侵入性代谢成为可能 在大型动物身上的研究。对老鼠、猫和恒河猴的研究表明 表明所有哺乳动物都具有伸长和脱饱和能力， 它可以在血液供应中被监测到。这种方法已经使 我们将研究酒精的影响的可能的人体试验 滥用和戒断以及饮食对脱饱和度和链长的影响。

Desaturation is a necessary step in the modification of essential fatty acids linoleic and linolenic acids to arachidonic and docosahexaenoic acid, respectively. The levels of these fatty acids are lowered by ethanol consumption in blood cells, liver and brain. The mechanisms underlying the disruption of essential fatty acid levels in these tissues is largely unknown. One possible explanation for this alcohol-induced deficiency is inhibition of elongation/desaturation of 18-carbon essential fatty acids by alcohol. A stable isotope gas chromatography/mass spectrometry (GC/MS) method has been developed that allows for the in vivo examination of these processes. This technique has improved the sensitivity of the method by several orders of magnitude and has made possible non-invasive metabolic studies in large animals. Studies in rats, cats and rhesus monkeys have indicated that all mammals have elongation and desaturation capability and that it may be monitored in the blood supply. This approach has made possible human trials in which we will investigate the effects of alcohol abuse and withdrawal as well as diet on desaturation and chain elongation.