SYSTEM SOFTWARE FOR PROTEIN AND NUCLEIC ACID STRUCTURE ANALYSIS

程序的这一部分的目的是使 普通生物医学界各种显微镜控制和 开发的图像采集/分析程序 一节。该计划是基于定量显微镜专家拥有的 可作为PC/AT(或完全兼容)的最低系统提供给他 MS-DOS机器)，具有最小512 KB内存和标准 30兆硬盘。所有与显微镜的最终对接 将通过市售接口卡实现 它们具有适当的模拟和数字信号I/O。图像 收购硬件也将是商业型的。我们计划 在公共领域中放置一系列源程序，编写 在C中(在PC世界中实现最大的可移植性)，这将允许 要逐步执行更多样和更复杂的功能 在计算机显微控制下获取的图像。程序 用户可以使用哪个将取决于有多少额外 他拥有的资源超过了规定的最低限度。在功能上 举例来说，控制程序将同样适用 好的到倒置显微镜(打开组织培养到 受控图像采集)。自由条款正在制定中 用于控制附加步进电机功能的软件 可由用户定义(例如冷凝器焦点、液体流动 进出房间等)。它计划包括 使用PC进行交互式图像处理的可能性 兼容高分辨率显示器，具有灰度级能力。 此选项和其他选项可根据用户兴趣提供 以及特定PC上可用的资源级别 有问题的。

The object of this part of the program is to make available to the general biomedical community the various microscope control and image acquisition/ analysis procedures developed within the section. The plan is based on the quantitative microscopist having available to him as a minimum system a PC/AT (or a fully compatible MS-DOS machine) with a minimum of 512 Kb of memory and a standard 30 megabyte hard disk. All final interfacing with the microscope will be accomplished via commercially available interface cards which have the appropriate analog and digital signal I/O. Image acquisition hardware will also be of the commercial type. We plan to place in the public domain a series of source programs, written in C (for maximum portability in the PC world) which will allow incrementally more varied and complex functions to be performed on images acquired under computer microscopic control. The procedures which the user can employ will depend upon how much additional resources he has available beyond the noted minimum. Functionally speaking, the programs for control, will for example, apply equally well to inverted microscopy (opening up tissue cultures to controlled image acquisition). Liberal provision is being made in the software for control of additional stepping motor functions which could be user defined (e.g. condenser focus, flow of liquids into and out of a chamber, etc). It is planned to include the possibility for interactive image processing, employing a PC compatible high resolution display with gray level capability. This and other options are available as a function of user interest and the level of resources available on the particular PC or PCs in question.