How simple plastic surfaces can be recruited to the fight against contact transmission of SARS-CoV-2

迄今为止，严重急性呼吸综合征冠状病毒2(SARS-CoV-2)似乎很容易在空气中的飞沫中传播，并通过表面传播。早期的研究表明，与其他表面相比，病毒在某些表面上的传染性似乎更长。然而，目前尚不清楚表面化学在病毒在表面宿主外的生存、感染性和变性过程中扮演什么角色。表面化学在其上减少了环境中传染性病毒的传播的简单聚合物，将比目前可用的活性负载和蛋白质涂层产品具有优势，这些产品会随着时间的推移而耗尽和降解。本项目的目的是确定对病毒吸附、感染性和变性行为的影响：1)一系列商用手套和其他PPE表面，例如面罩，以便能够推荐使用哪种现有聚合物来实现最佳PPE；以及2)使用诺丁汉大学最近开发的高通量方法筛选新的聚合物表面，以选择用于PPE和接触传播控制的新聚合物。目的：1.量化在环境条件下对现有PPE塑料的病毒结合和灭活。利用微阵列上展示的文库，定量SARS-CoV-2病毒样颗粒(VLP)或含刺的SARS2假病毒(PV)与聚合物结合的大小和特异性。量化SARS-CoV-2病毒在载玻片或多孔板和其他个人防护设备材料(使用诺丁汉的新设施)上的吸附、传染性和变性。开发用于个人防护设备和更广泛用于感染保护控制表面的抗SARS-CoV-2聚合物。

To date, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) appears to spread easily in droplets in the air and via surfaces. Early work indicates that viruses appear to remain infectious longer on some surfaces compared to others. However, it is currently unclear what role the surface chemistry plays in the survival, infectivity and denaturation process of the virus outside a host on surfaces.Simple polymers, on which the surface chemistry reduces the transfer of infectious viruses in the ambient environment, would have the advantage over the currently available active-loaded and protein-coated products which deplete and degrade over time. The aims of this project are to identify the effect on the virus adsorption, infectivity and denaturation behaviours of; 1) a range of commercially available gloves and other PPE surfaces, e.g. visors, to enable a recommendation of which existing polymers should be employed for optimal PPE, and 2) screen novel polymer surfaces using high throughput methodologies for selection of virucidal performance recently developed at the University of Nottingham, to develop new polymers for PPE and contact transmission control.Objectives:1. Quantify viral binding and inactivation under ambient conditions to existing PPE plastics.2. Quantify the magnitude and specificity of SARS-CoV-2 virus-like particle (VLP) or SARS2 spike-containing pseudovirus (PV) binding to polymers using libraries presented on micro arrays.3. Quantify the adsorption, infectivity and denaturation of SARS-CoV-2 viruses bound to the scaled up 'top 10' polymers of interest on slide or multiwell-plate and other PPE materials (using new facility in Nottingham).4. Develop anti-SARS-CoV-2 polymers for PPE and more widely for infection protection control surfaces.